FastQCFastQC Report
Fri 10 Feb 2017
SRR2568565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385945
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22050.036.6320421
TCAACGC34900.036.0680664
GTATCAA36400.036.0270271
CAACGCA35800.035.1613275
ATCAACG37000.034.151493
AACGCAG38450.032.857396
TATCAAC41000.031.0552672
CTACGCG650.00584832829.227699
ACGCAGA46800.026.2846227
CGCAGAG47150.025.9887758
GTACATG73000.025.3841441
ACATGGG73600.023.4938033
TACATGG76350.023.4593872
CATGGGG47500.021.7004414
TATAGCG2056.8342524E-720.854085
GTATAGC11650.020.8000811
GCAGAGT60650.020.282289
TTAATAC20500.019.9284863
TAATACT20150.019.8018914
CTTAATA19550.018.2263152
Produced by FastQC (version 0.11.5)