FastQCFastQC Report
Fri 10 Feb 2017
SRR2568563_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568563_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265251
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC27550.12162007653897955No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17700.034.6379741
TACCCCG1106.9861926E-834.5632175
TCAACGC24850.033.4679034
ATCAACG25800.032.2369843
CAACGCA25850.032.1732065
GTATCAA27600.032.0287441
AACGCAG27750.030.1402826
CGCAGAG32550.025.40428
ACGCAGA32650.025.326957
TATCAAC34650.024.6891062
ACATGGG54850.024.174843
GTACATG59750.023.6240651
CCCGTAC1050.002189757822.6316783
TACATGG63100.021.2400632
CATGGGG31850.021.0390684
TAATACT24400.019.0876774
GCAGAGT44200.018.3857389
GTATATA21800.018.3129731
GTACCGT2352.5182871E-618.2000376
CTGTACG2403.0782976E-617.8212649
Produced by FastQC (version 0.11.5)