FastQCFastQC Report
Fri 10 Feb 2017
SRR2568563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265251
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC29440.12996352280608198No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT27000.11919208953003442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25050.035.283951
TCAACGC23900.033.191554
GGTATCA15400.032.3996661
CAACGCA24700.032.116525
ATCAACG25900.031.0021483
AACGCAG27350.029.0001986
ACGCAGA30500.025.8493777
CGCAGAG30700.025.5262748
TATCAAC31850.025.5094492
GTACATG54650.025.0423051
ACATGGG52300.023.1655883
TACATGG59200.021.589622
TAATACT23500.020.4156154
CATGGGG31500.020.3578624
CTATGCG2803.497553E-818.6584919
TTACACT20150.018.3877474
TTAATAC26000.018.0911713
CTTAATA23500.017.9943662
ATACTTC24450.017.871086
GAGTACA40400.017.6433831
Produced by FastQC (version 0.11.5)