FastQCFastQC Report
Fri 10 Feb 2017
SRR2568561_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568561_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224669
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27900.1254119152107572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.052.6731261
GTATCAA49400.048.388231
TCAACGC60100.038.9026834
ATCAACG60700.038.6755873
CAACGCA60800.038.2185865
TATCAAC63950.037.081622
AACGCAG64150.036.2935686
GTACATG100900.031.9328161
ACGCAGA75200.030.897357
TACATGG103400.030.4713482
ACATGGG102800.029.909553
CGCAGAG78800.029.3049018
CATGGGA48800.025.2213784
GCAGAGT92550.024.6938679
GTATAGG7500.024.0780351
CATGGGG57650.023.080634
TACACCG3600.022.4396345
AGAGTAC78100.020.56298310-11
ATGGGAG26450.019.9419065
CAGAGTA85600.019.45515610-11
Produced by FastQC (version 0.11.5)