FastQCFastQC Report
Fri 10 Feb 2017
SRR2568561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224669
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33100.14878617897763668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.1214113200660413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28950.053.3396031
GTATCAA53900.043.282041
TCAACGC64200.035.7355274
ATCAACG65450.035.0553933
CAACGCA66000.034.760925
AACGCAG68100.034.0346836
TATCAAC68800.033.4903342
GTACATG97900.031.1578391
TACATGG101050.029.28992
ACGCAGA79750.028.8246257
ACATGGG101400.028.2954333
CGCAGAG83700.027.2940928
CATGGGG56300.025.2261434
CATGGGA47100.022.8924884
GCAGAGT99600.022.6984739
GTGGTAT14600.022.129471
GTATAGC9650.020.6793521
GAGTACT41250.020.14944612-13
AGAGTAC83850.020.07993110-11
ATCGTAC1456.136955E-419.6606661
Produced by FastQC (version 0.11.5)