FastQCFastQC Report
Fri 10 Feb 2017
SRR2568560_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568560_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494887
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.044.2707981
GTATCAA38550.040.5544131
TCAACGC40100.036.8530854
CAACGCA42800.034.527555
AACGCAG43650.033.961326
ATCAACG44600.033.135393
TATCAAC48500.030.6668532
GTACATG80650.028.8707681
TACATGG83550.027.3567942
ACATGGG81550.027.153673
ACGCAGA53900.027.0627927
CGCAGAG56050.025.6856178
CATGGGG57150.024.1955014
GTATACG1100.002871653521.599511
GTCCTAC6450.020.628371
ATGGGGC28550.020.4714285
GCAGAGT70400.020.3159
AGAGTAC59650.018.63924810-11
GTATTAC6700.018.440181
ATATGCG1559.605686E-418.3936845
Produced by FastQC (version 0.11.5)