FastQCFastQC Report
Fri 10 Feb 2017
SRR2568560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494887
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20100.051.5469631
GTATCAA38450.042.397521
TCAACGC43650.036.7816544
CAACGCA45500.035.286145
ATCAACG46000.035.014283
AACGCAG46750.034.338536
TATCAAC50550.032.0557672
GTACATG82350.029.318571
ACGCAGA56100.028.615447
TACATGG83850.027.9393832
CGCAGAG58850.027.278278
ACATGGG82950.026.7487683
CATGGGG56550.023.603294
CCGTATG3351.8189894E-1221.2663569
GCAGAGT74400.021.0024329
AGAGTAC62800.018.79374710-11
ACCGTAT3054.5747583E-918.6865028
GTATTAA15700.018.4661
GCGCCGT1559.635828E-418.3851076
GAGTACA58300.018.0978531
Produced by FastQC (version 0.11.5)