FastQCFastQC Report
Fri 10 Feb 2017
SRR2568558_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568558_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821119
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25050.042.8732871
GTACATG68900.036.0028881
GGTATCA15950.035.454581
TCAACGC29750.035.1396834
ATCAACG30150.034.3601653
CAACGCA30400.034.2320335
TACATGG73500.033.361722
ACATGGG74800.032.2736553
AACGCAG32900.031.77356
CGTATAC600.00394298931.68073
TATCAAC33800.031.0714592
CATGGGG48350.028.1081164
ACGCAGA38650.026.9235847
CGCAGAG41200.024.911218
GTATAGG5650.023.5502571
GTATATA9700.022.0458491
GAGTACA47350.020.8751411
ATGGGGG23700.020.8519745
CATGGGA27750.020.7197534
TCTATAC7200.019.800443
Produced by FastQC (version 0.11.5)