FastQCFastQC Report
Fri 10 Feb 2017
SRR2568558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821119
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27900.043.6050151
GGTATCA17100.042.242361
TCAACGC32250.037.263934
ATCAACG32450.037.038333
CAACGCA32650.036.8074075
AACGCAG34800.034.3912246
TATCAAC36300.033.9008262
GTACATG65550.032.1167561
CGTATAC902.4117582E-531.6704563
ACGCAGA39800.030.0707197
TACATGG70700.029.9731662
ACATGGG71750.028.3378873
CGCAGAG42950.028.086458
TATACCG1301.0024507E-525.5771755
CATGGGG47550.023.8751054
GCAGAGT55500.021.0507939
GTATTAC3900.020.7150021
AGCGTAC1404.8376562E-420.3668521
TTACCGT1200.00476427819.791864
ATACCGC1456.1543466E-419.6521286
Produced by FastQC (version 0.11.5)