FastQCFastQC Report
Fri 10 Feb 2017
SRR2568556_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568556_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135606
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33500.044.4100231
TCAACGC35300.040.7951554
GGTATCA19650.040.637611
ATCAACG36950.039.2343833
CAACGCA37200.038.708815
AACGCAG39400.036.906666
TATCAAC40750.035.8090022
ACGCAGA46250.030.9274757
CGCAGAG47500.029.8134578
GTACATG63400.025.7899591
TACATGG63200.025.1194922
ACATGGG62700.024.5617283
GCAGAGT56950.024.4485669
TACACCG3000.023.761845
CATGGGG45050.019.6227044
GTGTAGC15950.018.7741951
AGAGTAC51750.018.73265510-11
CAGAGTA55800.018.69303710-11
GTATATA13050.018.5755251
TACCGTA1901.860187E-417.5079047
Produced by FastQC (version 0.11.5)