FastQCFastQC Report
Fri 10 Feb 2017
SRR2568556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135606
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.041.374811
GTATCAA35350.041.1391371
TCAACGC39600.036.228624
ATCAACG40000.035.7525943
CAACGCA40900.034.8448075
AACGCAG43250.033.0528266
TATCAAC44800.032.244652
ACGCAGA50950.027.9643867
CGCAGAG51400.027.8119568
GTACATG62250.026.0338381
TACATGG64500.024.5327722
ACATGGG61750.024.0059413
CGTATAC2002.0507287E-823.7558783
GCAGAGT63800.022.183189
GTATAGC10950.019.9648881
GTGTAGC15750.019.6135391
TGGACCG3254.8748916E-1019.0020335
GTGTAGG11250.018.1651551
AGAGTAC57250.018.12609110-11
CATGGGG47950.017.8329834
Produced by FastQC (version 0.11.5)