FastQCFastQC Report
Fri 10 Feb 2017
SRR2568554_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568554_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851392
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22470.12136813813606194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.052.804111
GTATCAA48300.044.5719221
TCAACGC59250.035.6126984
ATCAACG61750.034.170893
AACGCAG63750.033.469786
CAACGCA63350.033.4569785
TATCAAC64500.032.9350282
ACGCAGA72300.029.1830967
GTACATG101000.029.0318051
TACATGG101400.028.4954722
ACATGGG99800.028.1904133
CGCAGAG74900.028.0424168
GTATACG1501.0560925E-625.3459741
ACCGTCG950.001223853725.0104438
GTATAGG7800.024.9804061
GCAGAGT85400.024.5945789
CATGGGA41350.024.0204454
CCTATAC7600.023.136543
CATGGGG49700.022.3753434
ATAGGAC6150.021.6368053
Produced by FastQC (version 0.11.5)