FastQCFastQC Report
Fri 10 Feb 2017
SRR2568554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851392
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28080.15166966261061948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23110.1248249965431416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.056.350641
GTATCAA48050.045.4948841
TCAACGC61450.035.402224
ATCAACG64600.033.830323
CAACGCA64600.033.6024255
TATCAAC65550.033.272042
AACGCAG66450.032.6651546
GTACATG102100.029.2767641
TACATGG103000.028.5095732
ACATGGG101450.028.3324473
ACGCAGA76900.027.8556737
CGCAGAG79050.026.7375538
CATGGGG52100.025.1627644
GCAGAGT90150.023.2346619
CGTATAG2354.629328E-922.2445161
CATGGGA39200.020.7203464
AGAGTAC84850.020.15181410-11
CATGGGC25900.019.8066444
CAGAGTA86600.019.36066610-11
TTAGCGC1507.724289E-418.9997084
Produced by FastQC (version 0.11.5)