FastQCFastQC Report
Fri 10 Feb 2017
SRR2568552_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568552_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484653
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62050.041.8906361
GGTATCA34700.040.946151
TCAACGC69050.035.9218564
ATCAACG70700.035.0850833
CAACGCA71450.034.7801935
AACGCAG75500.033.541976
GTACATG150700.032.793781
TACATGG150500.032.0164262
TATCAAC80100.031.0270562
ACATGGG158750.029.6341743
ACGCAGA93850.026.731157
CATGGGG106950.026.391114
CGCAGAG94250.026.3149348
GTATATA24750.021.5037521
GCAGAGT117600.020.4839539
ATGGGGG54300.020.3011535
TATAAGA25750.019.7459452
GTATAGG14550.019.595651
AGAGTAC97750.019.0052310-11
GTATAAA26200.018.681311
Produced by FastQC (version 0.11.5)