FastQCFastQC Report
Fri 10 Feb 2017
SRR2568552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4484653
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37650.043.668181
GTATCAA66150.037.4968341
TCAACGC70200.033.2906344
ATCAACG72700.032.1497843
CAACGCA73200.031.9262685
AACGCAG74800.031.3026666
GTACATG149800.031.0545221
TACATGG151200.030.1026482
TATCAAC84700.028.1586152
ACATGGG158500.027.9040873
ACGCAGA92600.025.0803687
CATGGGG106150.024.6561784
CGCAGAG93700.024.5831878
GTATATA26900.024.5536751
GTATAAT29200.019.3650261
GTACCGT5950.019.1571246
TATAAGA27200.018.8645232
ATATAAG23200.018.0238951
AGAGTAC100550.017.9252710-11
GTATTAC12800.017.8190771
Produced by FastQC (version 0.11.5)