FastQCFastQC Report
Fri 10 Feb 2017
SRR2568551_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568551_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804088
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35100.037.505631
TCAACGC35750.035.8912284
CAACGCA36300.035.4776955
ATCAACG36150.035.3645253
GGTATCA20900.034.1089861
AACGCAG39150.033.0140536
TATCAAC44050.029.237972
CGCAGAG44900.028.5735138
ACGCAGA45450.028.4373447
GTACATG69500.024.001931
ACATGGG68600.023.0698053
TACATGG72150.022.791012
CATGGGG40300.021.2259944
GCAGAGT62350.019.8907099
GTATATA20800.018.9643781
GTATTAG13800.018.5968111
GTATATG18950.018.3078561
ATAATAC13050.018.2088853
TAATACT19000.018.008584
AGAGTAC53300.017.74075310-11
Produced by FastQC (version 0.11.5)