FastQCFastQC Report
Fri 10 Feb 2017
SRR2568551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804088
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36500.036.8460461
GGTATCA22350.036.571961
ATCAACG36750.035.030523
TCAACGC36750.035.026774
CAACGCA37650.034.1894765
AACGCAG40450.031.7014566
TATCAAC42900.030.3462752
ACGCAGA47400.026.6524567
CGCAGAG47350.026.3796928
GTACATG73150.025.8562811
TACATGG75250.024.3089542
ACATGGG73300.024.0438793
CATGGGG44800.020.8870144
GTATTAT13250.020.084891
GTATATA22000.019.2249241
GCGTAAT1250.006016937619.0089131
GTATAGC13450.018.3729271
GCAGAGT65250.018.3423069
GTATAAT21350.018.0295311
TAATACT20400.017.9286964
Produced by FastQC (version 0.11.5)