FastQCFastQC Report
Fri 10 Feb 2017
SRR2568550_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568550_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203723
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.035.9089661
GTATCAA25500.035.598331
TCAACGC26500.032.9958274
ATCAACG27200.032.3250583
CAACGCA28500.030.8449675
AACGCAG29450.029.525256
TACATGG52900.029.1987422
GTACATG55150.028.3522051
TATCAAC33000.027.3637832
ACATGGG54550.026.9215583
ACGCAGA34200.025.1489477
CGCAGAG35350.024.3308078
CATGGGG33450.024.0091974
TTACGCA1456.130069E-419.663934
GTGTAGC20550.019.1956441
CTATACG1250.00601735519.0084674
CGTATAC1350.00937282517.6024321
TAGGGCG1350.00937896417.600434
GTATTAC6500.017.5482711
GTATTAT8550.017.2318551
Produced by FastQC (version 0.11.5)