FastQCFastQC Report
Fri 10 Feb 2017
SRR2568550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203723
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.040.6423381
GTATCAA25650.039.6465451
TCAACGC27800.034.8583264
ATCAACG28500.034.1734923
CAACGCA28600.033.8832745
AACGCAG29700.032.781586
GTACATG52050.030.8585071
TACATGG51750.029.838232
TATCAAC33350.029.632452
ACATGGG52200.028.3964753
ACCGCGT856.414333E-427.9372468
ACGCAGA35400.027.3690327
CGCAGAG35850.026.760538
CATGGGG35500.022.212684
GTATATA19300.019.6975231
GCAGAGT51100.018.7742659
AGAGTAC41950.018.0576510-11
GTATAGC16450.017.9103781
GTCCTAC4800.017.8201031
TATAGCG1600.001189528817.8168662
Produced by FastQC (version 0.11.5)