FastQCFastQC Report
Fri 10 Feb 2017
SRR2568548_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568548_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT531.1931562359297614No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.9680324178298064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.6978838361098605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.5177847816298964No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT180.40522287257991896No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG160.36019810895992793No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT140.31517334533993696No Hit
GGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAAA130.2926609635299415No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN130.2926609635299415No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG120.27014858171994593No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG120.27014858171994593No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG120.27014858171994593No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC110.24763619990995048No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC110.24763619990995048No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA110.24763619990995048No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT110.24763619990995048No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA110.24763619990995048No Hit
GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA110.24763619990995048No Hit
TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA100.22512381809995496No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT100.22512381809995496No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG100.22512381809995496No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA100.22512381809995496No Hit
CTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGA90.20261143628995948No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC90.20261143628995948No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA80.18009905447996397No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC80.18009905447996397No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.18009905447996397No Hit
ATCCTACTCTCTACAAACACTTATTACAACCCAAGAACACATATGATTAC80.18009905447996397No Hit
GTACATGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACAT80.18009905447996397No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT80.18009905447996397No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC70.15758667266996848No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA70.15758667266996848No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT70.15758667266996848No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG70.15758667266996848No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA70.15758667266996848No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC70.15758667266996848No Hit
GCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAAT70.15758667266996848No Hit
GTCCATGACCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAGGT70.15758667266996848No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA70.15758667266996848No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC70.15758667266996848No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT70.15758667266996848No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT70.15758667266996848No Hit
ATGTAATGGTAGCTGTTGGTGGGCTAATATTTATTAATACTAGAGTAGCT70.15758667266996848No Hit
TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG70.15758667266996848No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA60.13507429085997297No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG60.13507429085997297No Hit
GGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT60.13507429085997297No Hit
CCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACA60.13507429085997297No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT60.13507429085997297No Hit
GTACATGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA60.13507429085997297No Hit
GTATATACCTGTAATAATAATGTTAATTCCTATAAGAATAATGGTAAAGT60.13507429085997297No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA60.13507429085997297No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG60.13507429085997297No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC60.13507429085997297No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG60.13507429085997297No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC60.13507429085997297No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA50.11256190904997748No Hit
GGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATT50.11256190904997748No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG50.11256190904997748No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT50.11256190904997748No Hit
ACACTACACATCAGATACAATAACAGCCTTTTCATCAGTAACACACATTT50.11256190904997748No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT50.11256190904997748No Hit
ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT50.11256190904997748No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC50.11256190904997748No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11256190904997748No Hit
GATAAAAGGATAATAGCTATGGTTACTTCATATGAAATTGTTTGGGCTAC50.11256190904997748No Hit
CTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGA50.11256190904997748No Hit
TCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAA50.11256190904997748No Hit
TAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGC50.11256190904997748No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT50.11256190904997748No Hit
GGCTAATATTTATTAATACTAGAGTAGCTCCTCCGATTAGGTGTATTAAT50.11256190904997748No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA50.11256190904997748No Hit
CCATAAAATTATTTATAAAAGAACCAATACGCCCTTTAACAACCTCTATA50.11256190904997748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11256190904997748No Hit
TTATTGCACCTACCCTATCACTCACACTAGCATTAAGTCTATGAGTTCCC50.11256190904997748No Hit
GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA50.11256190904997748No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG50.11256190904997748No Hit
GGCTTATATTATGGATCATATACATTTATAGAAACCTGAAACATTGGAGT50.11256190904997748No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50.11256190904997748No Hit
GGTATCAACGCAGAGTACATGGGATGACCCACCAAACTCATGCATATCAC50.11256190904997748No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC50.11256190904997748No Hit
GGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAAT50.11256190904997748No Hit
CCTCTATATCCTTATTTATTATTGCACCTACCCTATCACTCACACTAGCA50.11256190904997748No Hit
GGTTGAGATAAATCATATTATGGCTATGGGTCAGGCTGGCAGAAGTAATC50.11256190904997748No Hit
AATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTAT50.11256190904997748No Hit
TTGTTGGTCCATACGGCATTTTACAACCATTTGCAGACGCCATAAAATTA50.11256190904997748No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC50.11256190904997748No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA50.11256190904997748No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA50.11256190904997748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACA156.1847013E-495.04
TACACAT250.004696110357.0000045
ACACATC250.004696110357.0000046
AATTACG150.00999054247.562-63
ATTACGG150.00999054247.562-63
ATAAAAT150.00999054247.560-61
TAATTTA150.00999054247.580-81
Produced by FastQC (version 0.11.5)