FastQCFastQC Report
Fri 10 Feb 2017
SRR2568548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT501.125619090499775No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT461.0355695632597928No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT440.9905447996398019No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.5628095452498875No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG220.49527239981990095No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG200.45024763619990993RNA PCR Primer, Index 23 (96% over 30bp)
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA200.45024763619990993No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG190.42773525438991444No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG180.40522287257991896No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT170.3827104907699235RNA PCR Primer, Index 23 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT170.3827104907699235RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.3376857271499325No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT140.31517334533993696No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC130.2926609635299415No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.27014858171994593No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC120.27014858171994593No Hit
TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA110.24763619990995048No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT110.24763619990995048No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC110.24763619990995048No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG110.24763619990995048No Hit
TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG110.24763619990995048No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT110.24763619990995048No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA100.22512381809995496No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA100.22512381809995496No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG100.22512381809995496No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG100.22512381809995496No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG100.22512381809995496No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT100.22512381809995496No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA100.22512381809995496No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT90.20261143628995948RNA PCR Primer, Index 23 (96% over 28bp)
CACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTT90.20261143628995948No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.20261143628995948No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT90.20261143628995948No Hit
GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA90.20261143628995948No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT80.18009905447996397No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG80.18009905447996397No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC80.18009905447996397No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA80.18009905447996397No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA80.18009905447996397No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA70.15758667266996848No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT70.15758667266996848No Hit
ATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTC70.15758667266996848No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT70.15758667266996848No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA70.15758667266996848No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT70.15758667266996848No Hit
ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT70.15758667266996848No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC70.15758667266996848No Hit
ATCCTACTCTCTACAAACACTTATTACAACCCAAGAACACATATGATTAC70.15758667266996848No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA70.15758667266996848No Hit
GTCTATGAGTTCCCCTACCAATACCACACCCATTAATTAATTTAAACCTA70.15758667266996848No Hit
GTACATGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA70.15758667266996848No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA70.15758667266996848No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC60.13507429085997297No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA60.13507429085997297No Hit
TATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGC60.13507429085997297No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC60.13507429085997297No Hit
CCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCC60.13507429085997297No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13507429085997297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13507429085997297No Hit
ATTCTAGACCCACTAACAAAATATATAGCATACCCCTTCATCCTTCTCTC60.13507429085997297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13507429085997297No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA60.13507429085997297No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT60.13507429085997297No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13507429085997297No Hit
GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT60.13507429085997297No Hit
GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT60.13507429085997297No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC60.13507429085997297No Hit
ACCAATACCACACCCATTAATTAATTTAAACCTAGGGATTTTATTTATTT50.11256190904997748No Hit
CTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGT50.11256190904997748No Hit
CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC50.11256190904997748No Hit
GGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT50.11256190904997748No Hit
TCCCTTGACATCGTGCTTCACGATACATACTATGTAGTAGCCCATTTCCA50.11256190904997748No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA50.11256190904997748No Hit
ACTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTC50.11256190904997748No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG50.11256190904997748No Hit
CCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACA50.11256190904997748No Hit
TAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGC50.11256190904997748No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT50.11256190904997748No Hit
CTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCAT50.11256190904997748No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT50.11256190904997748No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG50.11256190904997748No Hit
GGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAAT50.11256190904997748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGG200.001939034471.252
GGTATCA352.4931933E-454.2857171
AGTGGGC150.00999054247.564-65
GGTTTTA150.00999054247.572-73
GAGTACA300.00965999547.51
GTATCAA458.615882E-442.2222252
GAGTACT353.8424732E-640.71428712-13
ACGCAGA500.001447100538.0000048
CGCAGAG500.001447100538.0000049
GTACTTT409.6135245E-635.62514-15
AACGCAG550.002311395734.5454527
TCAACGC550.002311395734.5454525
ATCAACG550.002311395734.5454524
CAACGCA550.002311395734.5454526
ACTTTTT452.1541699E-531.66666816-17
TATCAAC600.003541845131.6666683
CAGAGTA504.4250017E-528.50000210-11
AGTACTT350.00795469227.14285914-15
Produced by FastQC (version 0.11.5)