##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568548_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4442 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.78433138226024 34.0 31.0 34.0 31.0 34.0 2 32.856596127870326 34.0 31.0 34.0 31.0 34.0 3 32.82687978388113 34.0 31.0 34.0 31.0 34.0 4 36.29761368752814 37.0 37.0 37.0 35.0 37.0 5 36.30594326879784 37.0 37.0 37.0 35.0 37.0 6 36.374380909500225 37.0 37.0 37.0 35.0 37.0 7 36.338135974786134 37.0 37.0 37.0 35.0 37.0 8 36.338135974786134 37.0 37.0 37.0 35.0 37.0 9 38.11053579468708 39.0 39.0 39.0 37.0 39.0 10-11 38.110986042323276 39.0 39.0 39.0 36.0 39.0 12-13 38.14903196758217 39.0 39.0 39.0 37.0 39.0 14-15 39.576767221972084 41.0 40.0 41.0 37.0 41.0 16-17 39.53872129671319 41.0 39.0 41.0 36.5 41.0 18-19 39.65904997748762 41.0 40.0 41.0 37.0 41.0 20-21 39.59984241332733 41.0 40.0 41.0 37.0 41.0 22-23 39.45418730301666 41.0 39.5 41.0 36.0 41.0 24-25 39.467807294011706 41.0 39.5 41.0 36.0 41.0 26-27 39.31945069788384 41.0 39.0 41.0 35.5 41.0 28-29 39.18685276902296 41.0 39.0 41.0 35.0 41.0 30-31 38.985704637550654 41.0 39.0 41.0 35.0 41.0 32-33 38.73165240882486 40.5 38.0 41.0 35.0 41.0 34-35 38.54761368752814 40.5 38.0 41.0 35.0 41.0 36-37 38.47523638000901 40.0 38.0 41.0 35.0 41.0 38-39 38.28376857271499 40.0 38.0 41.0 34.5 41.0 40-41 38.12573165240883 40.0 38.0 41.0 34.0 41.0 42-43 37.98480414227825 40.0 38.0 41.0 33.5 41.0 44-45 37.80256641152634 40.0 38.0 41.0 33.0 41.0 46-47 37.4986492570914 40.0 38.0 41.0 32.5 41.0 48-49 37.37314272850068 40.0 37.5 41.0 32.0 41.0 50-51 37.130684376407025 40.0 37.0 41.0 31.0 41.0 52-53 36.92131922557407 40.0 37.0 41.0 30.5 41.0 54-55 36.760468257541646 40.0 36.5 41.0 29.5 41.0 56-57 36.427172444844665 40.0 36.0 41.0 28.5 41.0 58-59 36.30796938316074 40.0 36.0 41.0 28.0 41.0 60-61 36.25146330481765 40.0 36.0 41.0 27.0 41.0 62-63 35.9972985141828 39.5 35.0 41.0 26.5 41.0 64-65 35.59049977487618 39.0 35.0 41.0 24.5 41.0 66-67 35.45486267447096 39.0 35.0 41.0 23.0 41.0 68-69 34.9720846465556 39.0 35.0 41.0 20.5 41.0 70-71 34.55740657361549 37.5 35.0 40.5 19.5 41.0 72-73 34.194281855020265 37.0 35.0 39.5 18.5 41.0 74-75 33.77667717244485 37.0 35.0 39.0 18.0 41.0 76-77 31.807631697433585 34.5 32.0 37.0 14.0 39.0 78-79 32.85670868977938 36.0 34.0 37.5 15.0 39.0 80-81 32.76598379108509 36.0 34.0 37.0 15.5 39.0 82-83 32.50303917154435 35.0 34.0 37.0 16.0 39.0 84-85 32.05661864025214 35.0 34.0 36.0 17.0 38.0 86-87 31.814723097703737 35.0 34.0 36.0 12.0 37.0 88-89 31.579806393516435 35.0 34.0 36.0 13.0 37.0 90-91 31.430211616389013 35.0 33.5 36.0 13.5 36.5 92-93 31.2461728950923 35.0 33.0 35.0 10.5 36.0 94-95 31.123930661864026 35.0 33.0 35.0 10.5 36.0 96-97 30.85918505177848 35.0 33.0 35.0 10.0 36.0 98-99 30.85884736605133 35.0 33.0 35.0 10.0 36.0 100-101 30.161413777577668 34.5 31.0 35.0 10.0 35.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6722098214285737 1101 2 -0.3796502976190439 1101 3 0.5450768849206327 1101 4 -0.3290550595238031 1101 5 -0.40871775793649334 1101 6 0.6109747023809646 1101 7 0.5039806547618966 1101 8 0.47884424603174125 1101 9 0.7132688492063437 1101 10-11 0.7793030753968253 1101 12-13 0.7679315476190496 1101 14-15 1.467888144841261 1101 16-17 1.3380270337301567 1101 18-19 1.3253162202380864 1101 20-21 1.3098586309523839 1101 22-23 1.4834201388888957 1101 24-25 -0.06701388888887294 1101 26-27 1.3230592757936535 1101 28-29 1.2896391369047606 1101 30-31 1.74626736111113 1101 32-33 1.758457341269839 1101 34-35 1.2658544146825434 1101 36-37 1.2254402281746053 1101 38-39 1.892962549603169 1101 40-41 2.171695188492073 1101 42-43 2.460274057539671 1101 44-45 1.538355654761908 1101 46-47 1.6473586309523824 1101 48-49 1.9503720238095283 1101 50-51 1.284151785714279 1101 52-53 2.5198598710317555 1101 54-55 1.66319444444445 1101 56-57 1.089081101190473 1101 58-59 0.9183221726190638 1101 60-61 1.01362847222223 1101 62-63 0.432930307539678 1101 64-65 -0.0889136904761827 1101 66-67 0.7012586805555614 1101 68-69 1.316629464285704 1101 70-71 0.33810763888890705 1101 72-73 1.4241133432539712 1101 74-75 2.0068700396825463 1101 76-77 0.4461805555555465 1101 78-79 1.9675285218253933 1101 80-81 2.4901661706349145 1101 82-83 2.923784722222244 1101 84-85 2.983420138888892 1101 86-87 3.450372023809525 1101 88-89 3.7442460317460267 1101 90-91 3.2118303571428584 1101 92-93 4.177604166666658 1101 94-95 2.4759052579364997 1101 96-97 4.34059399801588 1101 98-99 4.725595238095238 1101 100-101 4.463839285714272 1102 1 -0.16112351190476204 1102 2 -0.8796502976190439 1102 3 0.045076884920632665 1102 4 0.6709449404761969 1102 5 -0.07538442460315764 1102 6 -0.05569196428569967 1102 7 0.5039806547618966 1102 8 -0.18782242063492305 1102 9 -1.2867311507936563 1102 10-11 0.4459697420634967 1102 12-13 0.2679315476190496 1102 14-15 -0.6987785218254103 1102 16-17 0.33802703373015675 1102 18-19 -0.17468377976191363 1102 20-21 -0.35680803571428044 1102 22-23 0.6500868055555671 1102 24-25 -2.067013888888873 1102 26-27 -0.26027405753967514 1102 28-29 0.456305803571432 1102 30-31 0.1629340277778013 1102 32-33 1.4251240079365104 1102 34-35 0.26585441468254345 1102 36-37 1.808773561507934 1102 38-39 2.3096292162698333 1102 40-41 0.5050285218254089 1102 42-43 1.6269407242063423 1102 44-45 1.038355654761908 1102 46-47 1.0640252976190538 1102 48-49 -1.8829613095238003 1102 50-51 -0.715848214285721 1102 52-53 -0.48014012896824454 1102 54-55 0.49652777777777857 1102 56-57 2.089081101190473 1102 58-59 -1.0816778273809362 1102 60-61 -6.653038194444434 1102 62-63 -6.900403025793658 1102 64-65 -8.338913690476183 1102 66-67 -3.7987413194444386 1102 68-69 -3.0167038690476318 1102 70-71 -2.661892361111093 1102 72-73 -2.9092199900793645 1102 74-75 -0.659796626984118 1102 76-77 -7.720486111111118 1102 78-79 -5.365804811507939 1102 80-81 -5.843167162698418 1102 82-83 -0.49288194444442013 1102 84-85 2.0667534722222207 1102 86-87 0.7837053571428605 1102 88-89 -3.5890873015873055 1102 90-91 -4.871502976190477 1102 92-93 -4.98906250000001 1102 94-95 -2.1074280753968324 1102 96-97 -4.992739335317452 1102 98-99 -4.691071428571426 1102 100-101 -5.952827380952392 1103 1 0.5722098214285722 1103 2 0.5203497023809547 1103 3 0.8450768849206298 1103 4 0.2709449404761983 1103 5 0.19128224206350808 1103 6 0.6109747023809646 1103 7 0.5039806547618966 1103 8 0.47884424603174125 1103 9 -0.08673115079365346 1103 10-11 0.3793030753968267 1103 12-13 0.7679315476190496 1103 14-15 1.0678881448412625 1103 16-17 1.138027033730154 1103 18-19 0.5253162202380892 1103 20-21 1.2098586309523824 1103 22-23 0.7834201388888928 1103 24-25 0.1329861111111299 1103 26-27 -0.2269407242063437 1103 28-29 -0.010360863095236539 1103 30-31 0.29626736111112706 1103 32-33 0.6584573412698376 1103 34-35 0.9658544146825463 1103 36-37 0.7754402281746025 1103 38-39 1.0429625496031747 1103 40-41 1.1716951884920732 1103 42-43 0.5602740575396723 1103 44-45 0.9383556547619065 1103 46-47 0.1973586309523796 1103 48-49 1.0503720238095298 1103 50-51 1.8841517857142804 1103 52-53 2.2198598710317583 1103 54-55 2.2631944444444443 1103 56-57 2.789081101190476 1103 58-59 2.918322172619064 1103 60-61 3.01362847222223 1103 62-63 3.132930307539681 1103 64-65 2.1610863095238173 1103 66-67 -1.5987413194444358 1103 68-69 -2.0833705357143018 1103 70-71 -2.46189236111109 1103 72-73 -3.17588665674603 1103 74-75 -2.3931299603174523 1103 76-77 -2.253819444444453 1103 78-79 -1.8324714781746074 1103 80-81 -2.609833829365087 1103 82-83 -1.2262152777777544 1103 84-85 0.33342013888889355 1103 86-87 -2.249627976190478 1103 88-89 -1.5557539682539705 1103 90-91 -0.23816964285714093 1103 92-93 -1.4223958333333435 1103 94-95 -1.8740947420635017 1103 96-97 -1.5594060019841187 1103 98-99 -1.4244047619047606 1103 100-101 -0.6861607142857267 1104 1 1.1722098214285737 1104 2 -0.2129836309523796 1104 3 0.711743551587297 1104 4 0.6709449404761969 1104 5 0.5912822420635067 1104 6 0.6109747023809646 1104 7 0.5039806547618966 1104 8 0.47884424603174125 1104 9 0.7132688492063437 1104 10-11 0.7793030753968253 1104 12-13 0.7679315476190496 1104 14-15 1.3012214781745897 1104 16-17 1.1713603670634853 1104 18-19 1.3253162202380864 1104 20-21 1.3098586309523839 1104 22-23 -3.6832465277777686 1104 24-25 -0.06701388888887294 1104 26-27 0.5730592757936535 1104 28-29 0.7896391369047606 1104 30-31 1.49626736111113 1104 32-33 1.0917906746031676 1104 34-35 1.4325210813492149 1104 36-37 2.308773561507934 1104 38-39 2.642962549603169 1104 40-41 2.005028521825402 1104 42-43 2.2936073908729995 1104 44-45 2.2050223214285793 1104 46-47 2.564025297619054 1104 48-49 2.783705357142864 1104 50-51 3.6174851190476076 1104 52-53 3.853193204365084 1104 54-55 3.8298611111111143 1104 56-57 3.9224144345238017 1104 58-59 4.584988839285728 1104 60-61 4.8469618055555586 1104 62-63 5.099596974206342 1104 64-65 5.327752976190489 1104 66-67 4.701258680555561 1104 68-69 4.64996279761904 1104 70-71 5.838107638888907 1104 72-73 4.757446676587307 1104 74-75 6.006870039682546 1104 76-77 5.4461805555555465 1104 78-79 4.134195188492065 1104 80-81 4.4901661706349145 1104 82-83 4.007118055555573 1104 84-85 1.7334201388888921 1104 86-87 3.617038690476189 1104 88-89 4.077579365079362 1104 90-91 3.9618303571428584 1104 92-93 2.8442708333333293 1104 94-95 2.5592385912698354 1104 96-97 2.6739273313492156 1104 98-99 3.975595238095238 1104 100-101 3.5471726190476005 1105 1 0.9222098214285737 1105 2 0.1203497023809561 1105 3 0.29507688492063266 1105 4 0.6709449404761969 1105 5 0.5912822420635067 1105 6 0.6109747023809646 1105 7 0.5039806547618966 1105 8 0.47884424603174125 1105 9 0.2132688492063437 1105 10-11 0.7793030753968253 1105 12-13 0.3929315476190496 1105 14-15 0.9678881448412611 1105 16-17 0.8380270337301567 1105 18-19 0.8253162202380864 1105 20-21 0.30985863095238386 1105 22-23 -2.0165798611111043 1105 24-25 0.18298611111112706 1105 26-27 0.8230592757936535 1105 28-29 1.1646391369047606 1105 30-31 0.6212673611111299 1105 32-33 1.883457341269839 1105 34-35 1.2658544146825434 1105 36-37 -0.1495597718253947 1105 38-39 0.642962549603169 1105 40-41 0.9216951884920732 1105 42-43 1.2102740575396709 1105 44-45 0.41335565476190794 1105 46-47 1.1473586309523824 1105 48-49 1.4503720238095283 1105 50-51 1.784151785714279 1105 52-53 1.1448598710317555 1105 54-55 2.16319444444445 1105 56-57 2.214081101190473 1105 58-59 1.4183221726190638 1105 60-61 3.01362847222223 1105 62-63 2.307930307539678 1105 64-65 2.6610863095238173 1105 66-67 2.5762586805555614 1105 68-69 -0.30837053571429607 1105 70-71 -3.911892361111093 1105 72-73 -4.200886656746029 1105 74-75 -2.9931299603174537 1105 76-77 -3.3038194444444535 1105 78-79 -4.532471478174607 1105 80-81 -3.3848338293650855 1105 82-83 -3.076215277777756 1105 84-85 -1.8915798611111079 1105 86-87 -2.549627976190475 1105 88-89 -2.3807539682539733 1105 90-91 -2.4131696428571416 1105 92-93 -3.072395833333342 1105 94-95 -2.1490947420635003 1105 96-97 -2.65940600198412 1105 98-99 -3.274404761904762 1105 100-101 -3.036160714285728 1106 1 1.1722098214285737 1106 2 1.120349702380956 1106 3 1.0450768849206327 1106 4 0.6709449404761969 1106 5 0.5912822420635067 1106 6 0.6109747023809646 1106 7 0.5039806547618966 1106 8 0.47884424603174125 1106 9 0.7132688492063437 1106 10-11 0.7793030753968253 1106 12-13 0.7679315476190496 1106 14-15 0.9678881448412611 1106 16-17 1.1713603670634853 1106 18-19 0.15864955357142208 1106 20-21 0.8098586309523839 1106 22-23 0.4834201388888957 1106 24-25 1.0996527777777914 1106 26-27 1.2397259424603178 1106 28-29 1.456305803571425 1106 30-31 1.49626736111113 1106 32-33 1.9251240079365104 1106 34-35 2.099187748015872 1106 36-37 2.4754402281746053 1106 38-39 1.9762958829364976 1106 40-41 2.3383618551587375 1106 42-43 2.2936073908729995 1106 44-45 2.2050223214285793 1106 46-47 1.3973586309523824 1106 48-49 3.1170386904761926 1106 50-51 3.284151785714279 1106 52-53 3.5198598710317555 1106 54-55 3.16319444444445 1106 56-57 4.2557477678571445 1106 58-59 3.418322172619064 1106 60-61 3.51362847222223 1106 62-63 3.5995969742063423 1106 64-65 4.327752976190482 1106 66-67 3.8679253472222257 1106 68-69 2.816629464285704 1106 70-71 3.5047743055555713 1106 72-73 2.0907800099206355 1106 74-75 2.840203373015882 1106 76-77 1.6128472222222179 1106 78-79 1.9675285218253933 1106 80-81 2.323499503968243 1106 82-83 2.5071180555555728 1106 84-85 2.233420138888892 1106 86-87 3.450372023809525 1106 88-89 3.7442460317460267 1106 90-91 3.6284970238095227 1106 92-93 4.177604166666658 1106 94-95 4.059238591269828 1106 96-97 4.007260664682544 1106 98-99 4.142261904761902 1106 100-101 3.8805059523809433 1107 1 -2.627790178571427 1107 2 -2.879650297619044 1107 3 -2.754923115079368 1107 4 -3.9290550595238045 1107 5 -5.208717757936494 1107 6 -0.5890252976190382 1107 7 -0.4960193452381034 1107 8 -1.7211557539682616 1107 9 -1.0867311507936535 1107 10-11 -2.6206969246031733 1107 12-13 -2.0320684523809476 1107 14-15 -3.1321118551587404 1107 16-17 -2.061972966269842 1107 18-19 -1.274683779761908 1107 20-21 -2.690141369047616 1107 22-23 -1.0165798611111043 1107 24-25 -0.3670138888888701 1107 26-27 0.07305927579365346 1107 28-29 0.5896391369047578 1107 30-31 -4.403732638888876 1107 32-33 -5.741542658730161 1107 34-35 -2.7341455853174566 1107 36-37 -0.4245597718253933 1107 38-39 0.5429625496031747 1107 40-41 -0.8283048115079268 1107 42-43 -0.03972594246032912 1107 44-45 0.03835565476190794 1107 46-47 0.1973586309523796 1107 48-49 0.5503720238095227 1107 50-51 -1.4158482142857238 1107 52-53 -0.6801401289682474 1107 54-55 -3.2368055555555486 1107 56-57 -3.6109188988095298 1107 58-59 -1.981677827380942 1107 60-61 -2.8863715277777757 1107 62-63 -2.1670696924603163 1107 64-65 -2.13891369047618 1107 66-67 -1.2987413194444386 1107 68-69 -1.5833705357142946 1107 70-71 -0.06189236111109153 1107 72-73 -1.375886656746026 1107 74-75 -2.5931299603174516 1107 76-77 -1.8538194444444507 1107 78-79 -2.2324714781746096 1107 80-81 -1.5098338293650855 1107 82-83 -2.4262152777777573 1107 84-85 -1.066579861111105 1107 86-87 -1.7496279761904745 1107 88-89 -1.4557539682539726 1107 90-91 -1.4381696428571402 1107 92-93 -1.1223958333333428 1107 94-95 -1.574094742063501 1107 96-97 -1.7594060019841216 1107 98-99 -1.024404761904762 1107 100-101 -0.2861607142857281 1108 1 0.42220982142857366 1108 2 0.3703497023809561 1108 3 0.29507688492063266 1108 4 0.6709449404761969 1108 5 0.09128224206350666 1108 6 0.6109747023809646 1108 7 0.5039806547618966 1108 8 0.47884424603174125 1108 9 -0.2867311507936563 1108 10-11 0.5293030753968253 1108 12-13 0.2679315476190496 1108 14-15 0.8428881448412611 1108 16-17 0.8380270337301567 1108 18-19 -0.2996837797619136 1108 20-21 -0.8151413690476161 1108 22-23 -0.8915798611111043 1108 24-25 -0.44201388888887294 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2312 28-29 -2.3103608630952337 2312 30-31 -0.30373263888886726 2312 32-33 -0.6415426587301667 2312 34-35 -1.2341455853174566 2312 36-37 -1.7245597718253975 2312 38-39 -0.15703745039682815 2312 40-41 -0.12830481150792394 2312 42-43 0.4602740575396709 2312 44-45 -0.5616443452380935 2312 46-47 1.3973586309523824 2312 48-49 -0.8496279761904688 2312 50-51 -0.9158482142857238 2312 52-53 1.8198598710317526 2312 54-55 2.7631944444444443 2312 56-57 -1.1109188988095298 2312 58-59 0.5183221726190581 2312 60-61 0.41362847222223564 2312 62-63 1.7329303075396751 2312 64-65 2.4610863095238145 2312 66-67 2.101258680555567 2312 68-69 2.316629464285704 2312 70-71 1.9381076388889085 2312 72-73 2.824113343253977 2312 74-75 0.20687003968254913 2312 76-77 1.1461805555555493 2312 78-79 2.5675285218253947 2312 80-81 0.4901661706349145 2312 82-83 1.1737847222222442 2312 84-85 2.433420138888895 2312 86-87 2.6503720238095276 2312 88-89 2.544246031746024 2312 90-91 1.361830357142857 2312 92-93 2.5776041666666636 2312 94-95 3.6259052579365054 2312 96-97 0.7405939980158784 2312 98-99 0.37559523809523654 2312 100-101 0.6138392857142705 2313 1 -0.16112351190476204 2313 2 0.1203497023809561 2313 3 -0.9549231150793673 2313 4 0.004278273809532607 2313 5 0.25794890873017096 2313 6 -0.05569196428569967 2313 7 -0.6626860119047677 2313 8 0.14551091269840555 2313 9 0.5466021825396794 2313 10-11 0.1126364087301539 2313 12-13 0.2679315476190496 2313 14-15 -0.03211185515873893 2313 16-17 -0.9119729662698433 2313 18-19 0.4919828869047578 2313 20-21 0.14319196428571246 2313 22-23 -0.18324652777776862 2313 24-25 0.09965277777779136 2313 26-27 0.07305927579365346 2313 28-29 0.2896391369047606 2313 30-31 -0.003732638888870099 2313 32-33 -1.491542658730161 2313 34-35 -1.3174789186507851 2313 36-37 -2.0245597718253947 2313 38-39 -1.5237041170634953 2313 40-41 -5.244971478174598 2313 42-43 -2.623059275793665 2313 44-45 -0.8783110119047635 2313 46-47 -4.519308035714282 2313 48-49 -10.549627976190472 2313 50-51 -8.715848214285721 2313 52-53 -8.646806795634912 2313 54-55 -11.670138888888886 2313 56-57 -13.994252232142859 2313 58-59 -12.331677827380936 2313 60-61 -11.903038194444434 2313 62-63 -12.067069692460322 2313 64-65 -12.505580357142847 2313 66-67 -11.132074652777774 2313 68-69 -9.766703869047632 2313 70-71 -9.161892361111093 2313 72-73 -11.242553323412697 2313 74-75 -9.576463293650786 2313 76-77 -8.303819444444454 2313 78-79 -10.282471478174607 2313 80-81 -9.093167162698421 2313 82-83 -12.159548611111092 2313 84-85 -11.183246527777772 2313 86-87 -10.299627976190475 2313 88-89 -10.255753968253973 2313 90-91 -10.371502976190474 2313 92-93 -6.572395833333342 2313 94-95 -7.7740947420635 2313 96-97 -10.492739335317456 2313 98-99 -11.024404761904762 2313 100-101 -10.119494047619064 2314 1 -0.11350446428571104 2314 2 -1.0225074404761862 2314 3 -1.240637400793652 2314 4 -0.1861979166666572 2314 5 0.01985367063493726 2314 6 0.03954613095239523 2314 7 -1.3531622023809575 2314 8 -0.9497271825396894 2314 9 -0.0010168650793715983 2314 10-11 0.3507316468253947 2314 12-13 0.4822172619047649 2314 14-15 -0.889254712301593 2314 16-17 -0.44768725198413506 2314 18-19 0.11103050595237107 2314 20-21 -2.190141369047616 2314 22-23 -1.945151289682535 2314 24-25 -0.42415674603173414 2314 26-27 -1.3555121527777771 2314 28-29 -2.496075148809524 2314 30-31 -2.646589781746009 2314 32-33 -2.0272569444444457 2314 34-35 -0.5198598710317412 2314 36-37 0.6182973710317441 2314 38-39 -1.1427517361111157 2314 40-41 -4.042590525793642 2314 42-43 -2.9682973710317597 2314 44-45 -0.31878720238095326 2314 46-47 -0.8169270833333258 2314 48-49 -0.5496279761904717 2314 50-51 -1.215848214285721 2314 52-53 -0.19442584325395273 2314 54-55 0.09176587301588057 2314 56-57 -0.6966331845238187 2314 58-59 2.275465029761918 2314 60-61 2.870771329365091 2314 62-63 3.5043588789682474 2314 64-65 -2.3389136904761827 2314 66-67 -0.4415984623015774 2314 68-69 2.173772321428565 2314 70-71 3.480964781746046 2314 72-73 2.709827628968256 2314 74-75 1.292584325396831 2314 76-77 2.874751984126977 2314 78-79 2.3246713789682545 2314 80-81 3.4901661706349145 2314 82-83 2.173784722222244 2314 84-85 1.4477058531746003 2314 86-87 1.9503720238095248 2314 88-89 0.8156746031745961 2314 90-91 2.8189732142857196 2314 92-93 3.3204613095237967 2314 94-95 -0.27409474206350026 2314 96-97 -1.230834573412693 2314 98-99 0.6184523809523839 2314 100-101 0.4995535714285566 2315 1 0.33887648809523796 2315 2 0.1203497023809561 2315 3 0.8784102182539684 2315 4 0.004278273809532607 2315 5 0.25794890873017096 2315 6 0.6109747023809646 2315 7 0.17064732142856087 2315 8 -0.35448908730159445 2315 9 0.379935515873008 2315 10-11 0.5293030753968253 2315 12-13 0.7679315476190496 2315 14-15 1.0512214781745897 2315 16-17 1.1713603670634853 2315 18-19 0.908649553571415 2315 20-21 0.9765252976190553 2315 22-23 1.0667534722222243 2315 24-25 0.09965277777779136 2315 26-27 0.9897259424603249 2315 28-29 1.0396391369047606 2315 30-31 -0.3370659722221987 2315 32-33 1.008457341269839 2315 34-35 1.5158544146825434 2315 36-37 1.9754402281746053 2315 38-39 2.142962549603169 2315 40-41 1.3383618551587446 2315 42-43 1.9602740575396709 2315 44-45 1.8716889880952436 2315 46-47 3.0640252976190467 2315 48-49 2.8670386904761997 2315 50-51 2.6174851190476076 2315 52-53 3.186526537698427 2315 54-55 1.91319444444445 2315 56-57 2.7557477678571445 2315 58-59 1.6683221726190638 2315 60-61 3.26362847222223 2315 62-63 3.2662636408730066 2315 64-65 3.494419642857153 2315 66-67 4.28459201388889 2315 68-69 5.1499627976190325 2315 70-71 4.754774305555571 2315 72-73 4.424113343253971 2315 74-75 4.840203373015882 2315 76-77 5.779513888888875 2315 78-79 4.300861855158722 2315 80-81 4.906832837301586 2315 82-83 4.923784722222244 2315 84-85 1.7334201388888921 2315 86-87 3.367038690476189 2315 88-89 4.327579365079355 2315 90-91 4.711830357142858 2315 92-93 4.427604166666658 2315 94-95 4.309238591269828 2315 96-97 4.507260664682544 2315 98-99 4.642261904761909 2315 100-101 2.2971726190476076 2316 1 -0.32779017857142634 2316 2 0.2870163690476204 2316 3 -0.12158978174603163 2316 4 0.004278273809532607 2316 5 -0.07538442460315764 2316 6 0.2776413690476289 2316 7 0.3373139880952323 2316 8 0.47884424603174125 2316 9 0.7132688492063437 2316 10-11 0.5293030753968253 2316 12-13 -2.4820684523809504 2316 14-15 -0.03211185515873893 2316 16-17 0.504693700396821 2316 18-19 -0.09135044642857792 2316 20-21 -0.27347470238094473 2316 22-23 -0.34991319444444 2316 24-25 0.34965277777779846 2316 26-27 -3.5102740575396822 2316 28-29 -0.4603608630952394 2316 30-31 -2.50373263888887 2316 32-33 -3.3248759920634967 2316 34-35 -3.150812251984128 2316 36-37 -4.774559771825395 2316 38-39 -3.0237041170634953 2316 40-41 -2.494971478174598 2316 42-43 -4.4563926091270005 2316 44-45 -3.8783110119047635 2316 46-47 -4.685974702380953 2316 48-49 -2.3829613095238003 2316 50-51 -1.215848214285721 2316 52-53 -0.31347346230157314 2316 54-55 -3.33680555555555 2316 56-57 -2.7442522321428626 2316 58-59 -2.081677827380936 2316 60-61 -2.5697048611111057 2316 62-63 -1.067069692460322 2316 64-65 -0.8389136904761827 2316 66-67 -1.6320746527777672 2316 68-69 -1.433370535714296 2316 70-71 -1.7452256944444287 2316 72-73 -1.7425533234127002 2316 74-75 -0.659796626984118 2316 76-77 -0.8871527777777892 2316 78-79 -1.8658048115079424 2316 80-81 -1.7598338293650855 2316 82-83 -1.1595486111110915 2316 84-85 -1.5999131944444436 2316 86-87 -1.0496279761904752 2316 88-89 -0.0057539682539733406 2316 90-91 -0.8715029761904773 2316 92-93 -0.6557291666666742 2316 94-95 0.7259052579364997 2316 96-97 -0.07607266865078444 2316 98-99 -0.27440476190476204 2316 100-101 1.213839285714272 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 3.0 16 4.0 17 18.0 18 29.0 19 31.0 20 21.0 21 50.0 22 49.0 23 53.0 24 44.0 25 57.0 26 59.0 27 68.0 28 67.0 29 65.0 30 70.0 31 51.0 32 79.0 33 84.0 34 90.0 35 181.0 36 261.0 37 707.0 38 1704.0 39 597.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.85994145462734 21.977032199954966 14.658860616978158 21.50416572843954 2 14.343616302634544 23.62080612474668 42.220220671020044 19.815356901598737 3 13.088533453480514 31.83149357963505 35.43590898851093 19.64406397837351 4 8.036920306168392 20.4862674470959 41.91805493021162 29.558757316524087 5 7.181449797388565 41.08509680324178 38.991445294912204 12.742008104457453 6 21.769473210265648 44.416929311121116 20.531292210715893 13.282305267897343 7 23.21026564610536 34.96172895092301 26.78973435389464 15.038271049076993 8 24.35839711841513 37.61819000450248 22.714993246285456 15.308419630796937 9 24.60603331832508 13.529941467807294 23.30031517334534 38.56371004052229 10-11 20.4975236380009 27.9941467807294 33.55470508779829 17.95362449347141 12-13 22.512381809995496 27.40882485366952 31.34849167041873 18.730301665916254 14-15 20.823953174245837 30.79693831607384 27.36380009004953 21.015308419630795 16-17 18.448896893291312 31.573615488518687 30.290409725348944 19.687077892841064 18-19 18.78658262044124 30.042773525438992 31.382260243133725 19.788383610986042 20-21 19.664565511031068 29.62629446195407 32.30526789734354 18.40387212967132 22-23 19.259342638451148 30.346690679873934 31.596127870328683 18.79783881134624 24-25 19.05673120216119 31.14588023412877 31.573615488518687 18.223773075191353 26-27 17.964880684376407 32.06888788833859 32.440342188203516 17.525889239081494 28-29 18.077442593426383 28.45565060783431 33.577217469608286 19.889689329131023 30-31 18.64025213867627 30.729401170643854 32.440342188203516 18.190004502476363 32-33 18.730301665916254 30.650607834308868 34.275101305718145 16.343989194056732 34-35 18.21251688428636 29.95272399819901 34.08374606033318 17.75101305718145 36-37 19.20306168392616 29.885186852769024 32.316524088248535 18.595227375056282 38-39 17.728500675371453 31.29221071589374 33.273300315173344 17.705988293561457 40-41 16.591625393966684 29.153534443944167 36.954074741107604 17.30076542098154 42-43 17.728500675371453 30.66186402521387 32.32778027915354 19.281855020261144 44-45 17.89734353894642 30.335434488968932 32.980639351643404 18.78658262044124 46-47 18.111211166141377 28.804592525889237 33.34083746060333 19.743358847366054 48-49 16.861773975686628 30.290409725348944 33.307068887888335 19.540747411076094 50-51 17.886087348041425 29.75011256190905 32.687978388113464 19.675821701936062 52-53 17.09815398469158 30.706888788833858 32.654209815398474 19.540747411076094 54-55 18.887888338586222 30.93201260693381 32.316524088248535 17.863574966231425 56-57 18.685276902296263 30.977037370553806 30.391715443493922 19.94597028365601 58-59 17.570914002701485 30.312922107158936 32.85682125168843 19.259342638451148 60-61 18.178748311571365 33.723547951373256 29.885186852769024 18.21251688428636 62-63 16.974335884736604 34.62404322377308 31.629896443043677 16.771724448446644 64-65 17.73975686627645 30.718144979738852 32.29401170643854 19.24808644754615 66-67 18.268797838811345 32.733003151733456 30.346690679873934 18.651508329581272 68-69 18.021161638901397 33.352093651508326 30.66186402521387 17.964880684376407 70-71 19.84466456551103 29.79513732552904 32.11391265195858 18.246285457001353 72-73 18.80909500225124 31.75371454299865 29.074741107609185 20.362449347140927 74-75 17.53714542998649 32.316524088248535 30.740657361548852 19.405673120216118 76-77 18.35884736605133 30.920756416028816 32.496623142728495 18.223773075191353 78-79 17.71724448446646 30.740657361548852 31.708689779378656 19.833408374606034 80-81 17.28950923007654 32.429085997298515 30.042773525438992 20.238631247185953 82-83 18.60648356596128 30.020261143628996 31.438541197658715 19.934714092751012 84-85 16.86367218282112 31.48711021051447 30.023640661938533 21.62557694472588 86-87 17.503376857271498 33.39711841512832 30.84196307969383 18.257541647906347 88-89 17.886087348041425 30.729401170643854 32.136425033768575 19.24808644754615 90-91 17.54840162089149 31.35974786132373 31.652408824853666 19.439441692931112 92-93 18.70778928410626 32.77802791535345 29.660063034669065 18.85411976587123 94-95 18.20126069338136 30.110310670868977 30.54930211616389 21.139126519585773 96-97 19.732102656461052 30.369203061683926 29.76136875281405 20.13732552904097 98-99 18.74155785682125 29.491220171094103 31.91130121566862 19.85592075641603 100-101 17.817321770469647 29.992116229305104 31.737808311746818 20.45275368847843 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 3.0 2 4.0 3 7.0 4 6.0 5 3.5 6 4.0 7 11.0 8 11.5 9 14.5 10 17.0 11 9.0 12 6.5 13 9.0 14 12.0 15 9.0 16 5.0 17 10.5 18 12.0 19 8.0 20 9.0 21 11.0 22 10.0 23 12.5 24 16.5 25 17.5 26 16.5 27 18.0 28 26.0 29 37.0 30 62.5 31 85.0 32 132.5 33 204.5 34 261.0 35 282.5 36 322.0 37 352.5 38 319.0 39 301.5 40 257.5 41 208.5 42 185.5 43 164.5 44 159.5 45 136.5 46 105.0 47 90.5 48 87.5 49 73.5 50 61.5 51 50.5 52 37.0 53 25.5 54 15.0 55 18.5 56 20.5 57 19.0 58 16.0 59 10.5 60 8.0 61 7.0 62 5.0 63 5.5 64 4.0 65 2.0 66 1.0 67 0.0 68 0.5 69 1.0 70 1.0 71 0.5 72 0.5 73 0.5 74 0.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022512381809995496 2 0.022512381809995496 3 0.06753714542998648 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.011256190904997748 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.05628095452498874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4442.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.19585772174696 #Duplication Level Percentage of deduplicated Percentage of total 1 78.1767955801105 50.96803241782981 2 13.052486187845306 17.019360648356596 3 3.69475138121547 7.226474561008554 4 2.244475138121547 5.853219270598829 5 0.5179558011049723 1.6884286357496625 6 0.5179558011049723 2.0261143628995946 7 0.4488950276243094 2.0486267447095905 8 0.17265193370165746 0.9004952723998199 9 0.17265193370165746 1.0130571814497973 >10 1.0013812154696133 11.256190904997748 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50 1.125619090499775 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 46 1.0355695632597928 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 44 0.9905447996398019 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25 0.5628095452498875 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 22 0.49527239981990095 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 20 0.45024763619990993 RNA PCR Primer, Index 23 (96% over 30bp) CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 20 0.45024763619990993 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 19 0.42773525438991444 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 18 0.40522287257991896 No Hit ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 17 0.3827104907699235 RNA PCR Primer, Index 23 (96% over 25bp) ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 17 0.3827104907699235 RNA PCR Primer, Index 23 (95% over 23bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.3376857271499325 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 14 0.31517334533993696 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 13 0.2926609635299415 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.27014858171994593 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 12 0.27014858171994593 No Hit TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA 11 0.24763619990995048 No Hit GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT 11 0.24763619990995048 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 11 0.24763619990995048 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 11 0.24763619990995048 No Hit TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG 11 0.24763619990995048 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 11 0.24763619990995048 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 10 0.22512381809995496 No Hit GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA 10 0.22512381809995496 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 10 0.22512381809995496 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 10 0.22512381809995496 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 10 0.22512381809995496 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 10 0.22512381809995496 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 10 0.22512381809995496 No Hit CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT 9 0.20261143628995948 RNA PCR Primer, Index 23 (96% over 28bp) CACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTT 9 0.20261143628995948 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.20261143628995948 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 9 0.20261143628995948 No Hit GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA 9 0.20261143628995948 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 8 0.18009905447996397 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 8 0.18009905447996397 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 8 0.18009905447996397 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 8 0.18009905447996397 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 8 0.18009905447996397 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 7 0.15758667266996848 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 7 0.15758667266996848 No Hit ATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTC 7 0.15758667266996848 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 7 0.15758667266996848 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 7 0.15758667266996848 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 7 0.15758667266996848 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 7 0.15758667266996848 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 7 0.15758667266996848 No Hit ATCCTACTCTCTACAAACACTTATTACAACCCAAGAACACATATGATTAC 7 0.15758667266996848 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 7 0.15758667266996848 No Hit GTCTATGAGTTCCCCTACCAATACCACACCCATTAATTAATTTAAACCTA 7 0.15758667266996848 No Hit GTACATGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA 7 0.15758667266996848 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 7 0.15758667266996848 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 6 0.13507429085997297 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 6 0.13507429085997297 No Hit TATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGC 6 0.13507429085997297 No Hit CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC 6 0.13507429085997297 No Hit CCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCC 6 0.13507429085997297 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.13507429085997297 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.13507429085997297 No Hit ATTCTAGACCCACTAACAAAATATATAGCATACCCCTTCATCCTTCTCTC 6 0.13507429085997297 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.13507429085997297 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 6 0.13507429085997297 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 6 0.13507429085997297 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.13507429085997297 No Hit GTTTTGGACTGGTAGTTAGAAGAATAAGTGGAATTATGTGAAGGGCTATT 6 0.13507429085997297 No Hit GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT 6 0.13507429085997297 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 6 0.13507429085997297 No Hit ACCAATACCACACCCATTAATTAATTTAAACCTAGGGATTTTATTTATTT 5 0.11256190904997748 No Hit CTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGT 5 0.11256190904997748 No Hit CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC 5 0.11256190904997748 No Hit GGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT 5 0.11256190904997748 No Hit TCCCTTGACATCGTGCTTCACGATACATACTATGTAGTAGCCCATTTCCA 5 0.11256190904997748 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 5 0.11256190904997748 No Hit ACTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTC 5 0.11256190904997748 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 5 0.11256190904997748 No Hit CCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACA 5 0.11256190904997748 No Hit TAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGC 5 0.11256190904997748 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 5 0.11256190904997748 No Hit CTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCAT 5 0.11256190904997748 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 5 0.11256190904997748 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 5 0.11256190904997748 No Hit GGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAAT 5 0.11256190904997748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.03376857271499325 0.0 36-37 0.0 0.0 0.0 0.045024763619991 0.0 38-39 0.0 0.0 0.0 0.045024763619991 0.0 40-41 0.0 0.0 0.0 0.07879333633498425 0.0 42-43 0.0 0.0 0.0 0.12381809995497524 0.0 44-45 0.0 0.0 0.0 0.19135524538496174 0.0 46-47 0.0 0.0 0.0 0.22512381809995496 0.0 48-49 0.0 0.0 0.0 0.24763619990995048 0.0 50-51 0.0 0.0 0.0 0.3039171544349392 0.0 52-53 0.0 0.0 0.0 0.37145429986492573 0.0 54-55 0.0 0.0 0.0 0.49527239981990095 0.0 56-57 0.0 0.0 0.0 0.5965781179648807 0.0 58-59 0.0 0.0 0.0 0.7091400270148582 0.0 60-61 0.0 0.0 0.0 0.7316524088248537 0.0 62-63 0.0 0.0 0.0 0.7879333633498424 0.0 64-65 0.0 0.0 0.0 0.8554705087798289 0.0 66-67 0.0 0.0 0.0 0.9230076542098153 0.0 68-69 0.0 0.0 0.0 0.9905447996398018 0.0 70-71 0.0 0.0 0.0 1.0580819450697883 0.0 72-73 0.0 0.0 0.0 1.2156686177397569 0.0 74-75 0.0 0.0 0.0 1.35074290859973 0.0 76-77 0.0 0.0 0.0 1.485817199459703 0.0 78-79 0.0 0.0 0.0 1.5420981539846916 0.0 80-81 0.0 0.0 0.0 1.69968482665466 0.0 82-83 0.0 0.0 0.0 1.8910400720396219 0.0 84-85 0.0 0.0 0.0 2.206213417379559 0.0 86-87 0.0 0.0 0.0 2.3638000900495273 0.0 88-89 0.0 0.0 0.0 2.465105808194507 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATAGG 20 0.0019390344 71.25 2 GGTATCA 35 2.4931933E-4 54.285717 1 AGTGGGC 15 0.009990542 47.5 64-65 GGTTTTA 15 0.009990542 47.5 72-73 GAGTACA 30 0.009659995 47.5 1 GTATCAA 45 8.615882E-4 42.222225 2 GAGTACT 35 3.8424732E-6 40.714287 12-13 ACGCAGA 50 0.0014471005 38.000004 8 CGCAGAG 50 0.0014471005 38.000004 9 GTACTTT 40 9.6135245E-6 35.625 14-15 AACGCAG 55 0.0023113957 34.545452 7 TCAACGC 55 0.0023113957 34.545452 5 ATCAACG 55 0.0023113957 34.545452 4 CAACGCA 55 0.0023113957 34.545452 6 ACTTTTT 45 2.1541699E-5 31.666668 16-17 TATCAAC 60 0.0035418451 31.666668 3 CAGAGTA 50 4.4250017E-5 28.500002 10-11 AGTACTT 35 0.007954692 27.142859 14-15 >>END_MODULE