FastQCFastQC Report
Fri 10 Feb 2017
SRR2568544_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568544_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928691
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35000.042.9063841
GGTATCA19900.042.507911
TCAACGC38750.036.7919164
CAACGCA39800.035.6990855
ATCAACG39850.035.6570783
AACGCAG41600.034.1526376
TATCAAC43650.032.661782
TATACCG1601.899025E-929.6994925
ACGCAGA48200.029.4761377
CGCAGAG49650.028.4224178
GCAGAGT58200.023.7571249
GTACATG57850.023.4944651
TACATGG61300.021.1643542
ACATGGG59650.020.9530943
GTATATA16550.019.5260221
AGAGTAC52550.019.21365710-11
GTATAGA8600.018.788121
GTGTAGC11500.018.1826731
GTACCGT1851.5179174E-417.9792986
GTATAGC9300.017.884961
Produced by FastQC (version 0.11.5)