FastQCFastQC Report
Fri 10 Feb 2017
SRR2568544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928691
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.043.8633231
GTATCAA33650.041.2345621
TCAACGC36750.037.0943684
ATCAACG38450.035.459823
CAACGCA38500.035.408265
AACGCAG40300.033.9428566
TATCAAC43700.031.311732
ACGCAGA47500.028.697847
CGCAGAG47950.028.4285138
GTACATG55950.027.177751
ACATGGG57800.025.8079073
TACATGG60850.024.5949542
GCAGAGT57350.023.2720059
CATGGGG32300.021.4700854
GTATAGG8250.021.311371
CATGGGA26150.021.0702514
TATATAC17400.019.1117213
AGAGTAC52150.018.89838410-11
CAGAGTA57800.017.79061510-11
GTATATA19000.017.5068511
Produced by FastQC (version 0.11.5)