FastQCFastQC Report
Fri 10 Feb 2017
SRR2568543_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568543_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119158
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC43690.14006985218446774No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18900.040.732311
TCAACGC38450.036.211224
CAACGCA40750.033.9319885
GTATCAA44050.033.4427221
ATCAACG42800.032.6429523
AACGCAG45050.030.7971926
TATCAAC51550.027.6553042
CGCAGAG51200.025.9843048
ACGCAGA53200.025.8116787
GTACATG92850.025.4366721
ACATGGG81550.024.8239863
TACATGG101150.021.4702032
TAATACT34200.021.119784
CTCGTCC9450.020.1118139
CCTCGTC9650.019.6949868
GCAGAGT67700.019.5109849
CCGTACG757.730011E-418.99864232-33
CATGGGG46300.018.7820074
CATGGGA33800.018.6985034
AGAGTAC55150.018.56627710-11
Produced by FastQC (version 0.11.5)