FastQCFastQC Report
Fri 10 Feb 2017
SRR2568543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119158
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC45130.14468648269821535No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT33520.10746489918112517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.041.824381
TCAACGC42900.035.432014
GTATCAA46850.034.7961651
CGGATCG550.00258185134.541229
CAACGCA44300.034.2050325
ATCAACG46600.032.7280123
AACGCAG48900.031.177166
TATCAAC57750.026.988462
CGCAGAG55700.026.688838
ACGCAGA57000.026.4967547
GTACATG91250.025.4696431
ACATGGG82550.024.8637853
TAATACT33500.024.1050014
CTTAATA30450.022.9396252
TTAATAC33150.021.7851523
TATCGCG550.002878683221.59103290-91
TACATGG105200.020.822912
CCTCGTC10150.020.5886128
GTGTACG2108.5692955E-720.3690171
CATGGGG45450.019.9619314
Produced by FastQC (version 0.11.5)