FastQCFastQC Report
Fri 10 Feb 2017
SRR2568542_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568542_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053834
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.045.4720421
GTATCAA35650.042.1163751
TCAACGC37450.039.076154
ATCAACG39050.037.475993
CAACGCA39850.036.8410845
AACGCAG40850.035.939226
TATCAAC46250.031.8473572
ACGCAGA47350.030.7046177
CGCAGAG48350.030.1678358
GTACATG71650.025.9952091
ACATGGG70850.024.2097763
GCAGAGT59250.024.0566489
TACATGG77050.023.7416822
AGAGTAC51600.020.94758610-11
CATGGGG39200.020.3627324
CATGGGA28500.019.5053544
TAATACT22650.018.8793544
CCCCTAT7450.018.495461
CAGAGTA58750.017.8321210-11
TATAAGA13700.017.6877692
Produced by FastQC (version 0.11.5)