FastQCFastQC Report
Fri 10 Feb 2017
SRR2568542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053834
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC25720.1252292054761972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.047.375931
GTATCAA34950.040.239121
TCAACGC38600.036.4243664
CAACGCA39150.035.912665
ATCAACG39350.035.6137543
AACGCAG40800.034.2249646
TATCAAC45100.031.3907262
ACGCAGA48650.028.6049147
CGCAGAG49450.027.8548
GTACATG71000.026.031261
ACCGTAT1701.12468115E-725.1448758
ACATGGG69200.024.5077023
TACATGG78500.022.513082
GCAGAGT61600.021.743249
CGTTTCG550.002880065721.58903318-19
CATGGGG38900.020.8801754
GACCGTA1604.9950824E-520.7794447
ATATAAG14000.020.3623141
GTATAAA18750.019.2582241
GTATTAT13150.019.1493511
Produced by FastQC (version 0.11.5)