FastQCFastQC Report
Fri 10 Feb 2017
SRR2568541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280226
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.043.930181
GTATCAA24400.039.3494451
ATCAACG25350.035.812323
TCAACGC25000.035.7391174
CAACGCA26200.033.9181565
AACGCAG27600.032.369856
TATCAAC30450.030.4385782
GTACATG37900.028.3430291
ACGCAGA31400.028.3011387
ACATGGG36550.027.699333
CGCAGAG32100.027.5359368
TACATGG37650.027.268792
CATGGGG22400.023.9748764
GCAGAGT38250.022.9834489
CCCTACG1100.002866661421.6050051
CGCGCGA550.002878546221.59061418-19
GTATTAC4000.021.3889561
GTATAGC7400.021.196261
TAGCCCT13300.021.0827064
CATGGGA14000.020.3680364
Produced by FastQC (version 0.11.5)