FastQCFastQC Report
Fri 10 Feb 2017
SRR2568541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280226
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.046.033731
GTATCAA23550.038.1457061
TCAACGC24050.036.346024
ATCAACG24500.035.880763
CAACGCA25850.033.815165
AACGCAG26150.033.5983926
TATCAAC27550.031.9134652
ACGCAGA29650.029.4721327
CGCAGAG30900.028.2798928
ACATGGG34600.024.033553
GTACATG37600.024.0182041
GCAGAGT36350.023.9092049
TACATGG36950.023.794752
TAGACCG1150.003720499320.6550545
TCTATAC6100.019.4744933
CAGAGTA36150.019.37769510-11
GTATAGA7650.018.6395151
TGGTATC9100.018.278922
GTACGCA1300.00755750818.2717764
GAGTACT20350.018.20323812-13
Produced by FastQC (version 0.11.5)