FastQCFastQC Report
Fri 10 Feb 2017
SRR2568538_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568538_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77914
Sequences flagged as poor quality0
Sequence length101
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGACATT19112.4527042636753342No Hit
GACTAAGACAGGTGATAATTTCCAGTCATAAGATATCTATGCAATCTGAA14471.8571758605642117No Hit
TAAGATATCTATGCAATCTGAAAAATAAATGCTGTCACATTTTCCACAAA12011.5414431296044357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8871.1384346843956157No Hit
TCTTTAACTAGAATGATCATATTATTCTGTGGTTCTTGTAATAATGTTGT8021.029340041584311No Hit
TATCTATGCAATCTGAAAAATAAATGCTGTCACATTTTCCACAAAAAGCT7941.019072310496188No Hit
GTTTAATGTAGGGACATTCTGTAACCACTACTGAGAACAGCGAGCTTTTT7420.9523320584233899No Hit
GTCATAAGATATCTATGCAATCTGAAAAATAAATGCTGTCACATTTTCCA7150.9176784660009754No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA7080.908694201298868No Hit
ATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGACATTCTGTA6940.8907256718946531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.835536617295993No Hit
GGAATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAAGATAT6480.831686218137947No Hit
GTAATAATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAA6250.8021664912595939No Hit
TAATAATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAAC5200.6674025207279821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.6122134661293221No Hit
GGTGATAATTTCCAGTCATAAGATATCTATGCAATCTGAAAAATAAATGC4410.5660086762327695No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4110.5275046846523089No Hit
GATCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGA3940.5056857560900481No Hit
TCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGACA3250.4171265754549888No Hit
TAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAAGATATCTAT3200.4107092435249121No Hit
ATGTAGGGACATTCTGTAACCACTACTGAGAACAGCGAGCTTTTTGTGGA3070.39402418050671256No Hit
GAATAAACCCTTTCCTCCCCAGCTTGCTTCTTGGTCATGATGATTGTGCA2760.3542367225402367No Hit
ATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAAGATATCTA2680.34396899145211385No Hit
GTCCATGCCTGGTATCCACGAAGGTCAGAAGAGCTTTAATCTAGAACTAA2540.32600046204789895No Hit
ATAATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAACAG2480.31829966373180685No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2430.3118823318017301No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2400.30803193264368406No Hit
GTCTTAGTCAGGGTTTCTATTCCTGCACAATCATCATGACCAAGAAGCAA2330.2990476679415766No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2270.2913468696254845No Hit
GTCATGATGATTGTGCAGGAATAGAAACCCTGACTAAGACAGGTGATAAT2200.28236260492337706No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2010.2579767435890854No Hit
ACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAGCATTTATTTTTCA2000.25669327720307006No Hit
TGATGATTGTGCAGGAATAGAAACCCTGACTAAGACAGGTGATAATTTCC1960.25155941165900864No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA1930.24770901250096258No Hit
ATATTACAATGTCCATGCCTGGTATCCACGAAGGTCAGAAGAGCTTTAAT1930.24770901250096258No Hit
GTACTGGATGGTTTGTGTATTAACTTGACACAGCTGGAGTTATCACAGAG1880.24129168057088585No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA1840.23615781502682445No Hit
GTGTAAGCTGAATAAACCCTTTCCTCCCCAGCTTGCTTCTTGGTCATGAT1790.2297404830967477No Hit
CATGATGATTGTGCAGGAATAGAAACCCTGACTAAGACAGGTGATAATTT1780.22845701671073232No Hit
TATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGACATTC1750.22460661755268632No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1710.21947275200862487No Hit
CTACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAGCATTTATTTTT1670.2143388864645635No Hit
ATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAACAGCGA1640.21048848730651745No Hit
GTAAAGAACATCCCTCCATGGCCTCTGCATCAGTTCCTGCTTTCTGACCT1640.21048848730651745No Hit
GCATAGATATCTTATGACTGGAAATTATCACCTGTCTTAGTCAGGGTTTC1620.20792155453448674No Hit
AGAATGATCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGT1610.20663808814847137No Hit
TTCTGTAACCACTACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAG1590.2040711553764407No Hit
ATCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGAC1560.20022075621839464No Hit
GTGCTGTACTGGATGGTTTGTGTATTAACTTGACACAGCTGGAGTTATCA1520.19508689067433324No Hit
GATATCTTATGACTGGAAATTATCACCTGTCTTAGTCAGGGTTTCTATTC1500.19251995790230253No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1470.1886695587442565No Hit
GTGCAGGAATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAA1460.18738609235824114No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1450.1861026259722258No Hit
GAATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAAGATATC1450.1861026259722258No Hit
ATTTGATACCTTAAAGTTTTTGTTTCAAGTGCTGTACTGGATGGTTTGTG1420.18225222681417974No Hit
GTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAACAGCGAGC1380.17711836127011835No Hit
CATAAGATATCTATGCAATCTGAAAAATAAATGCTGTCACATTTTCCACA1320.16941756295402624No Hit
TATTTAAGCTCAATTTGATACCTTAAAGTTTTTGTTTCAAGTGCTGTACT1290.1655671637959802No Hit
AACTAGAATGATCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTA1290.1655671637959802No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1260.16171676463793414No Hit
TTCTATTCCTGCACAATCATCATGACCAAGAAGCAAGCTGGGGAGGAAAG1250.16043329825191877No Hit
TCTGTAACCACTACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAGC1230.15786636547988808No Hit
GTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAACAGCGAGCTTT1220.15658289909387274No Hit
TCTCAGTAGTGGTTACAGAATGTCCCTACATTAAACACAACATTATTACA1220.15658289909387274No Hit
TTATATTACAATGTCCATGCCTGGTATCCACGAAGGTCAGAAGAGCTTTA1210.1552994327078574No Hit
GAATAATATGATCATTCTAGTTAAAGAAGGCTGGAGAGACGGCTCAGTGG1210.1552994327078574No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA1210.1552994327078574No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1200.15401596632184203No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1170.15016556716379598No Hit
CTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGACATTCTGTAACC1160.14888210077778063No Hit
TCATATTACTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGACA1150.14759863439176527No Hit
GTCCTGGGTTCTGTAAGAAAGTAAGCTGAGCAAGCCAGGGGAAGCGAGCC1130.14503170161973458No Hit
AATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAAGATATCT1120.14374823523371924No Hit
AGTGGGGAGGGTCCCTTGTGGGCGGTGCCACCCCTGGACTAGTAGTCCTG1100.14118130246168853No Hit
TTGTAATAATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAG1100.14118130246168853No Hit
TCTTATGACTGGAAATTATCACCTGTCTTAGTCAGGGTTTCTATTCCTGC1090.13989783607567316No Hit
TGATTGTGCAGGAATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGT1080.13861436968965782No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.13733090330364248No Hit
CTGTAACCACTACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAGCA1000.12834663860153503No Hit
TCCATGGCCTCTGCATCAGTTCCTGCTTTCTGACCTGTTTGAGTTCCAGT990.12706317221551966No Hit
ACATTAAACACAACATTATTACAAGAACCACAGAATAATATGATCATTCT980.12577970582950432No Hit
CATGAATATTTAAGCTCAATTTGATACCTTAAAGTTTTTGTTTCAAGTGC970.12449623944348898No Hit
TCTTGTAATAATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTG960.12321277305747363No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT950.12192930667145828No Hit
GATAATTTCCAGTCATAAGATATCTATGCAATCTGAAAAATAAATGCTGT950.12192930667145828No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG940.12064584028544292No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.12064584028544292No Hit
CCTTAAAGTTTTTGTTTCAAGTGCTGTACTGGATGGTTTGTGTATTAACT930.11936237389942757No Hit
AGTTAAAGAAGGCTGGAGAGACGGCTCAGTGGCCAAGAGGACATGTTGCT920.11807890751341223No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG920.11807890751341223No Hit
AATAAATGCTGTCACATTTTCCACAAAAAGCTCGCTGTTCTCAGTAGTGG910.11679544112739688No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA900.11551197474138153No Hit
CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT900.11551197474138153No Hit
GTTCTCAGTAGTGGTTACAGAATGTCCCTACATTAAACACAACATTATTA890.11422850835536616No Hit
GGTCAGAAGAGCTTTAATCTAGAACTAAAGTTATGGATGGCTGTGAATCA880.11294504196935082No Hit
GTGTATTAACTTGACACAGCTGGAGTTATCACAGAGGAGGAGCTTAGTTA860.11037810919732012No Hit
ATATCTATGCAATCTGAAAAATAAATGCTGTCACATTTTCCACAAAAAGC860.11037810919732012No Hit
AACCACTACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAGCATTTA850.10909464281130478No Hit
TGATCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGG850.10909464281130478No Hit
GTTTGAGTTCCAGTCCTGACTTCCTTTGGTGATGAACAGCAGAATGGAAA830.10652771003927407No Hit
CTTCTTGGTCATGATGATTGTGCAGGAATAGAAACCCTGACTAAGACAGG820.10524424365325873No Hit
TTTCCAGTCATAAGATATCTATGCAATCTGAAAAATAAATGCTGTCACAT820.10524424365325873No Hit
GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA820.10524424365325873No Hit
ACCCAGGACTACTAGTCCAGGGGTGGCACCGCCCACAAGGGACCCTCCCC780.10011037810919732No Hit
CAGGAATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAAGAT780.10011037810919732No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATG200.002054998271.35281
GTATAAC200.002054998271.35281
TATGGGA250.00497524657.082244
TATATGG250.00497524657.082242
TATAACA250.00497524657.082242
TAACTAG1950.053.6325535
TTAACTA1900.052.575754
AACTAGA1950.051.194716
TAGAATG2050.051.016339
ACTAGAA2050.048.6974037
TATTACT405.319089E-447.568534
CTAGAAT2200.047.537948
TCTTTAA2150.046.4622881
CTTTAAC2150.046.4622882
ATTTAAG459.5321867E-442.2559475
TTTAACT2400.041.6224673
ATGGGGG608.181014E-539.6149525
GAATATT500.001595218738.0548251
GTGTCTC250.001610293238.00591768-69
ACGTAGG250.001610293238.00591726-27
Produced by FastQC (version 0.11.5)