FastQCFastQC Report
Fri 10 Feb 2017
SRR2568538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77914
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT29143.740021048848731No Hit
ATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGACATT18682.3975152090766745No Hit
GACTAAGACAGGTGATAATTTCCAGTCATAAGATATCTATGCAATCTGAA16772.152373129347742No Hit
TAAGATATCTATGCAATCTGAAAAATAAATGCTGTCACATTTTCCACAAA11881.524758066586236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10301.3219703775958107No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT9181.1782221423620916RNA PCR Primer, Index 33 (95% over 22bp)
GTTTAATGTAGGGACATTCTGTAACCACTACTGAGAACAGCGAGCTTTTT8251.058859768462664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8211.0537259029186026No Hit
GTCATAAGATATCTATGCAATCTGAAAAATAAATGCTGTCACATTTTCCA7620.9780013861436969No Hit
TATCTATGCAATCTGAAAAATAAATGCTGTCACATTTTCCACAAAAAGCT7490.9613163231254973No Hit
TCTTTAACTAGAATGATCATATTATTCTGTGGTTCTTGTAATAATGTTGT7290.9356469954051903No Hit
TAATAATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAAC6100.7829144954693636No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA5720.7341427728007803No Hit
GTAATAATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAA5680.729008907256719No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT5630.7225915753266422RNA PCR Primer, Index 33 (95% over 24bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG5500.7059065123084426RNA PCR Primer, Index 33 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.7046230459224273No Hit
ATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGACATTCTGTA5260.6751033190440742No Hit
GATCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGA4960.6365993274636137No Hit
GGAATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAAGATAT4550.5839772056369844No Hit
GGTGATAATTTCCAGTCATAAGATATCTATGCAATCTGAAAAATAAATGC4440.5698590753908156No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4340.5570244115306621No Hit
ATGTAGGGACATTCTGTAACCACTACTGAGAACAGCGAGCTTTTTGTGGA3090.3965911132787432No Hit
TAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAAGATATCTAT3070.39402418050671256No Hit
GAATAAACCCTTTCCTCCCCAGCTTGCTTCTTGGTCATGATGATTGTGCA2880.36963831917242085No Hit
GTCATGATGATTGTGCAGGAATAGAAACCCTGACTAAGACAGGTGATAAT2860.36707138640039016No Hit
ATAATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAACAG2850.3657879200143748No Hit
ACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAGCATTTATTTTTCA2590.3324177939779757No Hit
GTCCATGCCTGGTATCCACGAAGGTCAGAAGAGCTTTAATCTAGAACTAA2560.3285673948199297No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2470.3170161973457915No Hit
GTCTTAGTCAGGGTTTCTATTCCTGCACAATCATCATGACCAAGAAGCAA2470.3170161973457915No Hit
TCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGACA2350.3016146007136073No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2220.2849295376954078No Hit
TGATGATTGTGCAGGAATAGAAACCCTGACTAAGACAGGTGATAATTTCC2150.2759452729933003No Hit
GTGTAAGCTGAATAAACCCTTTCCTCCCCAGCTTGCTTCTTGGTCATGAT2060.26439407551916216No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT2040.2618271427471315No Hit
GTACTGGATGGTTTGTGTATTAACTTGACACAGCTGGAGTTATCACAGAG2020.25926020997510074No Hit
ATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAAGATATCTA1960.25155941165900864No Hit
CTACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAGCATTTATTTTT1930.24770901250096258No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT1910.2451420797289319RNA PCR Primer, Index 33 (96% over 27bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1880.24129168057088585No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1820.23359088225479377No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1780.22845701671073232No Hit
ATTTGATACCTTAAAGTTTTTGTTTCAAGTGCTGTACTGGATGGTTTGTG1760.22589008393870164No Hit
ATATTACAATGTCCATGCCTGGTATCCACGAAGGTCAGAAGAGCTTTAAT1740.22332315116667095No Hit
AGAATGATCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGT1730.22203968478065558No Hit
GAATAATATGATCATTCTAGTTAAAGAAGGCTGGAGAGACGGCTCAGTGG1720.22075621839464024No Hit
GCATAGATATCTTATGACTGGAAATTATCACCTGTCTTAGTCAGGGTTTC1660.21305542007854814No Hit
GTAAAGAACATCCCTCCATGGCCTCTGCATCAGTTCCTGCTTTCTGACCT1610.20663808814847137No Hit
AATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAAGATATCT1610.20663808814847137No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1600.20535462176245606No Hit
TTCTGTAACCACTACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAG1600.20535462176245606No Hit
GTGCTGTACTGGATGGTTTGTGTATTAACTTGACACAGCTGGAGTTATCA1580.20278768899042532No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.20022075621839464No Hit
GTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAACAGCGAGCTTT1510.19380342428831787No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA1470.1886695587442565No Hit
GTGCAGGAATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGTCATAA1430.18353569320019508No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA1380.17711836127011835No Hit
GTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAACAGCGAGC1350.17326796211207227No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1350.17326796211207227No Hit
TCTTATGACTGGAAATTATCACCTGTCTTAGTCAGGGTTTCTATTCCTGC1320.16941756295402624No Hit
CTCCAGCCTTCTTTAACTAGAATGATCATATTATTCTGTGGTTCTTGTAA1280.16428369740996485No Hit
TCCATGGCCTCTGCATCAGTTCCTGCTTTCTGACCTGTTTGAGTTCCAGT1270.16300023102394948No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC1250.16043329825191877No Hit
AATAAATGCTGTCACATTTTCCACAAAAAGCTCGCTGTTCTCAGTAGTGG1230.15786636547988808No Hit
CATGATGATTGTGCAGGAATAGAAACCCTGACTAAGACAGGTGATAATTT1230.15786636547988808No Hit
GATATCTTATGACTGGAAATTATCACCTGTCTTAGTCAGGGTTTCTATTC1230.15786636547988808No Hit
ATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAGAACAGCGA1210.1552994327078574No Hit
CTAGAATGATCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAAT1200.15401596632184203No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA1170.15016556716379598RNA PCR Primer, Index 33 (96% over 29bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1170.15016556716379598No Hit
AACCACTACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAGCATTTA1160.14888210077778063No Hit
TGATTGTGCAGGAATAGAAACCCTGACTAAGACAGGTGATAATTTCCAGT1160.14888210077778063No Hit
ACATTAAACACAACATTATTACAAGAACCACAGAATAATATGATCATTCT1150.14759863439176527No Hit
GATAATTTCCAGTCATAAGATATCTATGCAATCTGAAAAATAAATGCTGT1150.14759863439176527No Hit
TTTCCAGTCATAAGATATCTATGCAATCTGAAAAATAAATGCTGTCACAT1150.14759863439176527No Hit
TCTGTAACCACTACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAGC1140.14631516800574992No Hit
TCTCAGTAGTGGTTACAGAATGTCCCTACATTAAACACAACATTATTACA1140.14631516800574992No Hit
TTATATTACAATGTCCATGCCTGGTATCCACGAAGGTCAGAAGAGCTTTA1130.14503170161973458No Hit
AACTAGAATGATCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTA1120.14374823523371924No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT1110.1424647688477039No Hit
TTCTATTCCTGCACAATCATCATGACCAAGAAGCAAGCTGGGGAGGAAAG1100.14118130246168853No Hit
TATTTAAGCTCAATTTGATACCTTAAAGTTTTTGTTTCAAGTGCTGTACT1100.14118130246168853No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA1100.14118130246168853No Hit
CTGTAACCACTACTGAGAACAGCGAGCTTTTTGTGGAAAATGTGACAGCA1090.13989783607567316No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC1080.13861436968965782No Hit
TATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTAGGGACATTC1080.13861436968965782No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1070.13733090330364248No Hit
GGTCAGAAGAGCTTTAATCTAGAACTAAAGTTATGGATGGCTGTGAATCA1050.1347639705316118No Hit
AGTGGGGAGGGTCCCTTGTGGGCGGTGCCACCCCTGGACTAGTAGTCCTG1020.13091357137356574No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1000.12834663860153503No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG990.12706317221551966RNA PCR Primer, Index 15 (95% over 24bp)
TCTTGTAATAATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTG980.12577970582950432No Hit
ATATCTATGCAATCTGAAAAATAAATGCTGTCACATTTTCCACAAAAAGC980.12577970582950432No Hit
CATGAATATTTAAGCTCAATTTGATACCTTAAAGTTTTTGTTTCAAGTGC970.12449623944348898No Hit
GTTCTCAGTAGTGGTTACAGAATGTCCCTACATTAAACACAACATTATTA970.12449623944348898No Hit
TTGTAATAATGTTGTGTTTAATGTAGGGACATTCTGTAACCACTACTGAG970.12449623944348898No Hit
CCTTAAAGTTTTTGTTTCAAGTGCTGTACTGGATGGTTTGTGTATTAACT960.12321277305747363No Hit
GTATTAACTTGACACAGCTGGAGTTATCACAGAGGAGGAGCTTAGTTAGG950.12192930667145828No Hit
CATAAGATATCTATGCAATCTGAAAAATAAATGCTGTCACATTTTCCACA940.12064584028544292No Hit
ATTATCACCTGTCTTAGTCAGGGTTTCTATTCCTGCACAATCATCATGAC920.11807890751341223No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.11551197474138153No Hit
GAATGATCATATTATTCTGTGGTTCTTGTAATAATGTTGTGTTTAATGTA890.11422850835536616No Hit
GTCCTGGGTTCTGTAAGAAAGTAAGCTGAGCAAGCCAGGGGAAGCGAGCC880.11294504196935082No Hit
AGTTAAAGAAGGCTGGAGAGACGGCTCAGTGGCCAAGAGGACATGTTGCT880.11294504196935082No Hit
GTTCTTTACTGGCTCGCTTCCCCTGGCTTGCTCAGCTTACTTTCTTACAG880.11294504196935082No Hit
GTTTGAGTTCCAGTCCTGACTTCCTTTGGTGATGAACAGCAGAATGGAAA860.11037810919732012No Hit
ACAATGTCCATGCCTGGTATCCACGAAGGTCAGAAGAGCTTTAATCTAGA830.10652771003927407No Hit
ATAATTTCCAGTCATAAGATATCTATGCAATCTGAAAAATAAATGCTGTC820.10524424365325873No Hit
GCAGAATGGAAAGTGTAAGCTGAATAAACCCTTTCCTCCCCAGCTTGCTT820.10524424365325873No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT820.10524424365325873RNA PCR Primer, Index 33 (95% over 21bp)
ACCCAGGACTACTAGTCCAGGGGTGGCACCGCCCACAAGGGACCCTCCCC820.10524424365325873No Hit
GTGTATTAACTTGACACAGCTGGAGTTATCACAGAGGAGGAGCTTAGTTA800.10267731088122803No Hit
CCCCTGGACTAGTAGTCCTGGGTTCTGTAAGAAAGTAAGCTGAGCAAGCC790.10139384449521266No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT780.10011037810919732No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA780.10011037810919732No Hit
CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT780.10011037810919732No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATT156.564851E-495.107971
ATGGGCA250.005006713356.9915245
GGAAGGC250.005006713356.9915248
GGTGGCG250.005006713356.9915249
GGGGCAG602.3161192E-855.4084247
GGGCAGT707.803101E-847.4929438
TTACAAT707.803101E-847.4929434
TGGGGCA707.803101E-847.4929436
TACAATG707.803101E-847.4929435
TAACTAG1200.047.4929355
TTAACTA1200.047.4929354
CTTTAAC1250.045.651822
TTTAACT1250.045.6225053
ATGGGGC857.0831447E-944.6992385
CATGGGG1650.043.17544
ATATTAT4600.042.385071
TCTTTAA1350.042.2702061
TATTATT4900.041.7310452
CTTATAC9200.041.351291
ATTATTC4900.040.734383
Produced by FastQC (version 0.11.5)