FastQCFastQC Report
Fri 10 Feb 2017
SRR2568536_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568536_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305136
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36750.039.5704231
GGTATCA23150.037.1564521
TCAACGC38600.035.5798384
ATCAACG40400.033.995633
GTACATG105100.033.6868321
CAACGCA40650.033.6670955
AACGCAG42100.032.5075386
TACATGG109950.031.1635322
ACATGGG108500.030.8791983
TATCAAC47850.029.1992742
CATGGGG65400.026.8854584
ACGCAGA53050.025.3501957
CGCAGAG55350.024.2964328
CATGGGA50400.021.4979864
GAGTACA72200.020.2072771
AGTACAT72000.018.5472892
GCAGAGT74800.017.5970049
GTATAGA15150.016.9391
ATGGGGG37600.016.9352115
AGAGTAC60500.016.92592610-11
Produced by FastQC (version 0.11.5)