FastQCFastQC Report
Fri 10 Feb 2017
SRR2568536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305136
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23150.038.3896831
GTATCAA39150.037.267521
TCAACGC39850.035.4015274
CAACGCA41500.034.222915
ATCAACG41250.034.206233
GTACATG104500.032.5627171
AACGCAG45000.031.9789966
TACATGG108550.030.6850382
ACATGGG104350.030.6404653
TATCAAC47600.029.7473162
ACGCAGA53500.026.6319077
CATGGGG66800.024.9588474
CGCAGAG57000.024.6633248
CATGGGA45650.021.5388974
GAGTACA70750.020.6222421
AGTACAT69700.019.0199722
GCAGAGT75550.018.5448069
GTAATAC8150.018.0707783
ATGGGGG33800.017.7071115
GTATAGT12250.017.0702761
Produced by FastQC (version 0.11.5)