FastQCFastQC Report
Fri 10 Feb 2017
SRR2568535_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568535_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4514744
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.033.992021
TCAACGC40450.033.718224
GTATCAA44350.033.5413781
CAACGCA42300.032.3548165
GTACATG120550.031.9336091
ATCAACG44900.030.4843063
ACATGGG116750.029.9606423
AACGCAG47100.029.1564626
TACATGG130150.028.5191822
CATGGGG75550.026.6076894
CGCAGAG55950.024.0350068
ACGCAGA56650.023.906047
TATCAAC59000.023.5212862
TAATACT40450.021.1473164
ATCGCGT1150.00371678820.6599868
GTATTAC15700.019.0708961
GTATTAT25900.018.5332581
ATTATAC36850.018.442883
GTATATA32950.018.3180181
GAGTACA84100.018.1400811
Produced by FastQC (version 0.11.5)