FastQCFastQC Report
Fri 10 Feb 2017
SRR2568535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4514744
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24700.036.3609051
TCAACGC45000.033.987624
GTATCAA49350.033.0275841
CAACGCA46850.032.5441445
ATCAACG50050.030.7535533
GTACATG118350.030.3545231
AACGCAG51400.029.7530636
ACATGGG115350.028.1292063
TACATGG130050.026.4152362
CATGGGG70750.026.182774
TATCAAC60950.025.413852
ACGCAGA59850.024.9174987
CGCAGAG61450.024.2687118
TAATACT39500.018.5182974
ATGGGGC34150.018.0813185
GTATAAT40800.017.8197291
GCAGAGT82700.017.803089
CAGTGCG5650.017.6526459
CTAATAC19700.017.6039873
GTATATA31600.017.5941641
Produced by FastQC (version 0.11.5)