FastQCFastQC Report
Fri 10 Feb 2017
SRR2568534_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568534_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362015
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32550.038.5470051
GGTATCA19500.036.5590061
TCAACGC35850.034.0693054
CAACGCA37000.033.136735
ATCAACG37650.032.3156363
AACGCAG39500.031.3997256
GTACATG72800.029.834761
TATCAAC43350.028.5050622
ACATGGG75950.027.4709993
TACATGG78650.027.3135052
ACGCAGA47650.026.0291527
CGCAGAG49150.024.944198
CATGGGG53100.024.6125984
TATACCG1503.0242914E-522.1767795
GCAGAGT64100.018.5334059
ACCGTAC1559.601903E-418.3947039
TGTACGC2152.3846083E-517.6843572
AGAGTAC56000.017.565310-11
ATGGGGG23750.017.2078445
CATGGGA25450.016.993114
Produced by FastQC (version 0.11.5)