FastQCFastQC Report
Fri 10 Feb 2017
SRR2568534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362015
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32600.041.546081
GGTATCA19350.038.067431
TCAACGC36850.036.2232744
CAACGCA37500.035.7220765
ATCAACG39500.033.798123
AACGCAG40750.032.866126
TATCAAC44850.030.2993182
GTACATG74000.029.6696971
TACATGG76150.028.1407622
ACGCAGA47900.027.7619157
ACATGGG77550.026.3432833
TATACCG1301.0022393E-525.5784115
CGCAGAG51750.025.512998
CATGGGG51800.023.5679744
ACCGCGC1100.0028808221.5875958
TATACTG12300.020.468675
GCAGAGT65600.019.8368939
GAGTACA53500.018.2097051
AGAGTAC56850.018.17003310-11
GTATATA18250.017.9675561
Produced by FastQC (version 0.11.5)