FastQCFastQC Report
Fri 10 Feb 2017
SRR2568531_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568531_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4099212
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC32850.032.8410534
ATCAACG33150.032.8317833
CAACGCA33450.032.2492145
GTATCAA37850.030.638431
AACGCAG37350.028.8793546
GTACATG88700.028.2912941
GGTATCA26300.028.0103441
ACATGGG85850.026.8499813
TACATGG93700.025.665722
TATCAAC48100.023.3189742
ACGCAGA46400.023.2472047
CGCAGAG46250.023.2198588
GTATATA27150.021.1815661
CATGGGG60300.020.964814
TATACTG24600.018.3517685
GTATTAT22750.017.3396051
TAATACT29550.016.887234
TATACCG3702.733941E-916.6967145
GAGTACA58400.016.6833611
TCTATAC21100.016.4430713
Produced by FastQC (version 0.11.5)