FastQCFastQC Report
Fri 10 Feb 2017
SRR2568531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4099212
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC33500.031.477184
ATCAACG35100.030.1816983
CAACGCA35050.030.0851785
GTATCAA37950.029.2977831
GGTATCA27100.029.280461
AACGCAG38800.027.4189596
GTACATG88900.027.0445331
ACATGGG92150.024.4359343
TACATGG94650.024.3449782
GTATATA27750.023.4578551
CGCAGAG45250.023.1957478
TATCAAC47400.022.8530852
ACGCAGA47000.022.3320757
CATGGGG59900.019.8244364
GAGTACA62000.018.162991
ATAATAC20600.017.5263823
GCACCGT6600.017.2703826
TAATACT31700.016.9319444
GTATTAG20000.015.9175231
GTATAAA24500.015.9029791
Produced by FastQC (version 0.11.5)