FastQCFastQC Report
Fri 10 Feb 2017
SRR2031868_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031868_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030122
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97090.3204161416603028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79670.2629267072414906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47000.15510926622756444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45100.058.348111
GTATCAA85900.042.5785831
TCAACGC99500.035.9472354
ATCAACG100700.035.5188673
CAACGCA102250.034.9804425
AACGCAG105300.034.0123446
TATCAAC107650.033.2698552
ACGCAGA117950.030.243757
CGCAGAG121300.029.4084918
GTACATG89550.025.354551
TACATGG88950.025.3119722
GCAGAGT137650.025.2597189
GAGTACT85850.023.6255112-13
ACATGGG92750.023.5067393
CAGAGTA130350.022.6112410-11
AGAGTAC122400.021.596210-11
CATGGGG60500.021.276864
GTACTTT97100.020.86380214-15
AGTACTT94350.018.87917112-13
GTATACG1801.2341664E-418.4722211
Produced by FastQC (version 0.11.5)