FastQCFastQC Report
Fri 10 Feb 2017
SRR2031868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030122
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117290.38708012416661775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100070.33025072917856113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57900.19108141520374428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46250.060.0663151
GTATCAA88350.043.343021
TCAACGC109100.035.2215274
ATCAACG109400.034.9512673
CAACGCA111000.034.490265
AACGCAG113300.033.9158746
TATCAAC114100.033.7197042
ACGCAGA127400.030.124957
CGCAGAG128350.029.8279618
GCAGAGT145850.025.8582129
GTACATG79750.025.2909431
TACATGG80150.024.9495662
GAGTACT100400.023.8204612-13
ACATGGG81400.023.4577333
CAGAGTA140150.022.80900810-11
AGAGTAC130550.022.5761210-11
CATGGGG51200.021.3374394
GTACTTT112400.020.98153914-15
AGTACTT108550.020.4348512-13
ATAACGC1600.001191795217.8121223
Produced by FastQC (version 0.11.5)