FastQCFastQC Report
Fri 10 Feb 2017
SRR2031867_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031867_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494992
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58770.3931124715048642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50190.335720860044736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32110.2147837580401768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.052.7197761
GTATCAA48500.041.9175261
TCAACGC58600.034.6928334
CAACGCA58850.034.384035
ATCAACG59650.034.0025143
AACGCAG62000.032.8669366
TATCAAC62100.032.661032
ACGCAGA68700.029.7307137
CGCAGAG68250.029.4395628
GCAGAGT75650.025.6807659
AGAGTAC65350.023.11400210-11
GAGTACT53700.022.9539112-13
TACATGG30250.022.1404952
GTACATG30800.022.0535721
CAGAGTA73750.021.60847510-11
AGTACTT58050.021.19293812-13
GTACTTT59250.020.56329214-15
ACATGGG31550.020.4754373
GTATAAT7450.017.2147661
TACTTTT71250.017.014-15
Produced by FastQC (version 0.11.5)