FastQCFastQC Report
Fri 10 Feb 2017
SRR2031867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494992
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70020.46836371030747986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60310.4034135299720667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38290.25612177188908036No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT23690.15846238642079688No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29600.054.4446751
GTATCAA54100.041.2119331
TCAACGC66250.033.5539864
ATCAACG66400.033.3351143
CAACGCA67100.032.987365
TATCAAC69200.032.0549352
AACGCAG69050.031.9869946
ACGCAGA74750.029.4843067
CGCAGAG74900.029.4252598
GCAGAGT83150.025.9344949
GAGTACT61650.024.03840612-13
GTACATG33050.023.7333971
TACATGG32450.023.7128892
AGAGTAC73400.023.23178310-11
CAGAGTA81450.022.36445810-11
GTACTTT68300.021.66314314-15
AGTACTT66000.021.51846912-13
ACATGGG34200.021.3884223
ATGACGG1404.8533897E-420.3566995
CATGGGG18800.019.7070164
Produced by FastQC (version 0.11.5)