FastQCFastQC Report
Fri 10 Feb 2017
SRR2031866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55180
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT11342.0550924247915914No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11031.9989126495106924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10001.812250815512867No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9831.7814425516491483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9721.7615077926785068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5651.0239217107647698No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.7828923523015585No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.5781080101486046No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT2730.4947444726350127RNA PCR Primer, Index 26 (100% over 22bp)
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.48930772018847407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.3624501631025734No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT1900.3443276549474447RNA PCR Primer, Index 26 (100% over 24bp)
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.2428416092787242No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.2029720913374411No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.17941283073577383No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA900.16310257339615802No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.16310257339615802No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC830.15041681768756796No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC810.14679231605654222No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG800.14498006524102935RNA PCR Primer, Index 26 (100% over 29bp)
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT730.13229430953243929No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT720.13048205871692642No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG720.13048205871692642No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA720.13048205871692642No Hit
TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT710.12866980790141355No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.1214208046393621No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT660.11960855382384922RNA PCR Primer, Index 26 (100% over 27bp)
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA660.11960855382384922No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG650.11779630300833635No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG620.11235955056179776No Hit
CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC620.11235955056179776No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA590.10692279811525915No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT590.10692279811525915No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA570.10329829648423343No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG570.10329829648423343No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAC156.585026E-494.997288
GATGACC200.002063824371.247969
CCACCTA200.002063824371.247968
ATGGGAT504.1687417E-756.9983675
CACCTAT250.00499659856.9983679
ATCCACC250.00499659856.9983676
GGTATCA5150.050.8650471
CGCAGAA707.740164E-847.498649
TTAACTG250.001609715437.9989160-61
GTATCAA7550.034.6016582
CATGGGA1800.034.3045734
GTTATTG300.003936170631.6657682-83
AACGCAG8800.029.6866517
GTATAAT650.005720611729.309651
CTTGAAA2650.028.67842556-57
CAACGCA9250.028.2424356
TTGAAAA2700.028.14734558-59
TCAACGC9350.027.9403765
TATCAAC9400.027.7917583
GACCCAC350.00836046427.14207812-13
Produced by FastQC (version 0.11.5)