##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031866_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55180 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70998550199348 34.0 31.0 34.0 31.0 34.0 2 32.83191373686118 34.0 33.0 34.0 31.0 34.0 3 32.813320043494016 34.0 33.0 34.0 31.0 34.0 4 36.257575208408845 37.0 37.0 37.0 35.0 37.0 5 36.284849583182314 37.0 37.0 37.0 35.0 37.0 6 36.41312069590431 37.0 37.0 37.0 35.0 37.0 7 36.37169264226169 37.0 37.0 37.0 35.0 37.0 8 36.399057629575935 37.0 37.0 37.0 35.0 37.0 9 38.158281986226896 39.0 39.0 39.0 37.0 39.0 10-11 38.181388184124685 39.0 39.0 39.0 37.0 39.0 12-13 38.198414280536426 39.0 39.0 39.0 37.0 39.0 14-15 39.6367524465386 41.0 40.0 41.0 37.0 41.0 16-17 39.53613628126132 41.0 39.0 41.0 37.0 41.0 18-19 39.560456687205516 41.0 39.5 41.0 36.5 41.0 20-21 39.547127582457406 41.0 40.0 41.0 36.0 41.0 22-23 39.40992207321493 41.0 39.0 41.0 35.0 41.0 24-25 39.289507067778175 41.0 39.0 41.0 35.0 41.0 26-27 39.09301377310619 41.0 38.5 41.0 35.0 41.0 28-29 38.95313519391084 41.0 38.0 41.0 35.0 41.0 30-31 38.68146067415731 40.0 37.5 41.0 35.0 41.0 32-33 38.006922798115255 40.0 35.0 41.0 34.5 41.0 34-35 37.923840159478075 40.0 35.0 41.0 35.0 41.0 36-37 37.78765857194636 40.0 35.0 41.0 35.0 41.0 38-39 37.52188292859732 40.0 35.0 41.0 34.0 41.0 40-41 37.39259695541863 40.0 35.0 41.0 33.5 41.0 42-43 37.11274012323305 40.0 35.0 41.0 33.0 41.0 44-45 36.700480246466114 40.0 35.0 41.0 32.0 41.0 46-47 36.28299202609641 40.0 35.0 41.0 29.5 41.0 48-49 35.91095505617977 40.0 35.0 41.0 26.0 41.0 50-51 35.702935846321125 40.0 35.0 41.0 23.5 41.0 52-53 35.7707593330917 40.0 35.0 41.0 23.0 41.0 54-55 35.62384015947807 40.0 35.0 41.0 22.0 41.0 56-57 35.418303733236684 40.0 35.0 41.0 20.0 41.0 58-59 35.17980246466111 39.0 35.0 41.0 19.0 41.0 60-61 34.84845958680681 39.0 35.0 41.0 16.5 41.0 62-63 34.4914371148967 39.0 35.0 41.0 14.5 41.0 64-65 34.17213664371149 38.0 35.0 41.0 11.5 41.0 66-67 33.77086806814063 37.0 35.0 40.5 10.0 41.0 68-69 33.183399782529904 37.0 34.5 39.5 10.0 41.0 70-71 32.66505074302283 36.0 34.0 39.0 9.0 41.0 72-73 32.183218557448356 36.0 34.0 39.0 8.0 41.0 74-75 31.77355019934759 35.0 33.0 37.5 8.0 40.0 76-77 30.482312432040594 34.5 31.0 36.5 8.0 39.0 78-79 31.11565784704603 35.0 33.0 37.0 8.0 39.0 80-81 30.955364262413916 35.0 33.0 36.0 8.0 39.0 82-83 30.65171257702066 35.0 33.0 36.0 8.0 37.0 84-85 30.455599855019933 35.0 33.0 35.5 7.5 37.0 86-87 30.20983146067416 35.0 32.0 35.0 7.0 36.5 88-89 29.992633200434938 35.0 32.0 35.0 7.0 36.0 90-91 29.8013320043494 35.0 31.5 35.0 7.0 36.0 92-93 29.660175788329106 35.0 31.0 35.0 7.0 36.0 94-95 29.533309169989128 35.0 31.0 35.0 7.0 36.0 96-97 29.443176875679594 35.0 31.0 35.0 7.0 35.0 98-99 29.372082276187026 35.0 31.0 35.0 7.0 35.0 100-101 28.503588256614716 34.0 28.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5050393725415958 1101 2 0.3775271841769552 1101 3 0.5457707998342372 1101 4 0.4955878891085561 1101 5 0.5133484718861823 1101 6 0.5365062460474164 1101 7 0.45419608867755557 1101 8 0.24430049691811462 1101 9 0.4494883013699891 1101 10-11 0.5095394493421566 1101 12-13 0.3944719923284268 1101 14-15 0.5117237761240716 1101 16-17 0.6319126829742743 1101 18-19 0.8696085668768276 1101 20-21 0.6904638538176044 1101 22-23 0.5402272474645002 1101 24-25 0.27450404473731993 1101 26-27 0.33284879610366147 1101 28-29 -0.23859745048272174 1101 30-31 -0.08446294044801306 1101 32-33 0.0418689917158801 1101 34-35 -0.7324797180834111 1101 36-37 -0.9305432356712373 1101 38-39 -0.17418325808218782 1101 40-41 -0.8720899845646528 1101 42-43 -2.202513659644083 1101 44-45 -2.6966390145671113 1101 46-47 -2.534147187823848 1101 48-49 -2.3559954767366307 1101 50-51 -1.6187670229838105 1101 52-53 -1.130139807579475 1101 54-55 -0.9443000539343913 1101 56-57 -1.2440499773949583 1101 58-59 -1.591959731963236 1101 60-61 -1.8759595715372939 1101 62-63 -1.722239451984784 1101 64-65 -1.277660865824899 1101 66-67 -2.383990998606258 1101 68-69 -1.9065653277148158 1101 70-71 -1.3583910108680328 1101 72-73 -1.1494055286887956 1101 74-75 -1.873472379160269 1101 76-77 -1.3706049520192174 1101 78-79 -1.701390460249879 1101 80-81 -1.708999699007581 1101 82-83 -2.5751056474424914 1101 84-85 -3.0038844905711812 1101 86-87 -2.319325510227479 1101 88-89 -2.4028399511617913 1101 90-91 -2.356165693182394 1101 92-93 -1.9346090639774793 1101 94-95 -2.2220924917152587 1101 96-97 -2.10403854274276 1101 98-99 -2.0871652525132234 1101 100-101 -2.910902443568613 1102 1 0.25171343019126624 1102 2 0.32264913539646756 1102 3 0.5252608220071906 1102 4 0.1618850066251838 1102 5 -0.10971139507612548 1102 6 -0.32047823288828425 1102 7 0.07670163191480128 1102 8 -0.120444514168355 1102 9 -0.0421968427541799 1102 10-11 -0.352711104981573 1102 12-13 -0.14516215401303612 1102 14-15 -0.36521635691362064 1102 16-17 -0.14663498886606874 1102 18-19 0.09715845601208883 1102 20-21 -0.30260709961920895 1102 22-23 0.3434423250698231 1102 24-25 0.5039940669102592 1102 26-27 -0.12252814402938128 1102 28-29 0.02609212823124807 1102 30-31 0.0222443766251601 1102 32-33 0.03299981211498704 1102 34-35 0.1319881311405382 1102 36-37 0.14567627652388637 1102 38-39 -0.12651141772742847 1102 40-41 0.6115574655462055 1102 42-43 -0.42230301662856107 1102 44-45 0.7315760630382044 1102 46-47 0.9350878454355751 1102 48-49 0.8421752549706909 1102 50-51 0.4876631322268352 1102 52-53 0.6747382412010126 1102 54-55 0.47282854916982586 1102 56-57 0.27147108690659394 1102 58-59 -0.03181560779471937 1102 60-61 -0.35905269792310435 1102 62-63 -0.8089911149559583 1102 64-65 -0.31784933589141673 1102 66-67 0.0018183140323202451 1102 68-69 0.009177466076756957 1102 70-71 -0.6280695031075041 1102 72-73 -0.8170884555180642 1102 74-75 -0.5128847960117078 1102 76-77 -1.2528111604449386 1102 78-79 -1.3626986642410124 1102 80-81 -0.5493544661916161 1102 82-83 -0.8303717228305132 1102 84-85 -1.001667195670958 1102 86-87 -0.27165366987271256 1102 88-89 -0.8801126784345179 1102 90-91 -1.0255115911868309 1102 92-93 -0.7311722568821395 1102 94-95 -1.1267488110057222 1102 96-97 -0.3191161480642677 1102 98-99 -0.9884956294533538 1102 100-101 -1.6354035522160615 1103 1 0.34493836244058684 1103 2 0.23863829528806946 1103 3 0.470518274581714 1103 4 0.34508283860350275 1103 5 0.07294443148214214 1103 6 0.18196079150196454 1103 7 0.30217588665735207 1103 8 0.11803787065549187 1103 9 0.41362971551139793 1103 10-11 0.33807480287750735 1103 12-13 0.45836088121730967 1103 14-15 0.563238927639226 1103 16-17 0.36095308701468554 1103 18-19 0.5597600820283546 1103 20-21 0.39576688412063277 1103 22-23 0.6407322979695564 1103 24-25 0.6381404083736797 1103 26-27 0.6785558668107328 1103 28-29 0.47731164042637175 1103 30-31 0.20164817066309837 1103 32-33 -0.10358555373866807 1103 34-35 0.342015231411537 1103 36-37 0.294456764328757 1103 38-39 -0.11458729848622795 1103 40-41 0.4291726416979671 1103 42-43 0.6790519969215723 1103 44-45 1.0177549248268178 1103 46-47 1.2307518020751473 1103 48-49 0.9578934121522522 1103 50-51 1.0155764113596248 1103 52-53 0.6587490813094163 1103 54-55 0.8104979258635936 1103 56-57 0.9993843660393864 1103 58-59 1.4860705710670672 1103 60-61 1.2485353779576585 1103 62-63 1.1855888308434999 1103 64-65 1.3826926695286375 1103 66-67 1.3766150619998 1103 68-69 1.1116164904670072 1103 70-71 1.7605483830713595 1103 72-73 1.1533722490889815 1103 74-75 1.5984973178094286 1103 76-77 1.2417687853545196 1103 78-79 1.203154994295577 1103 80-81 0.9647376747297933 1103 82-83 1.4135307161938755 1103 84-85 1.5213680346813447 1103 86-87 1.418048227146258 1103 88-89 1.753220654898815 1103 90-91 1.5248949128782137 1103 92-93 1.4517545723861538 1103 94-95 1.5859883163655475 1103 96-97 1.7575776188734018 1103 98-99 1.1976832323352653 1103 100-101 1.3683904857243157 1104 1 0.06136348321353324 1104 2 0.4536141406986971 1104 3 0.39853759825321333 1104 4 0.0924258337725874 1104 5 0.08647100153044818 1104 6 0.2558738349802212 1104 7 0.04806960646411085 1104 8 0.2798736194477627 1104 9 0.46628672034231755 1104 10-11 0.2827607932156724 1104 12-13 0.14990677493712212 1104 14-15 0.19947081169719638 1104 16-17 -0.2786604395553667 1104 18-19 0.589470226955882 1104 20-21 0.5377958696278782 1104 22-23 0.8455632158439457 1104 24-25 0.7577056257649843 1104 26-27 0.61792784748706 1104 28-29 0.6947029447741997 1104 30-31 0.681841407378073 1104 32-33 0.8007622723482868 1104 34-35 0.5002277917980109 1104 36-37 0.6727176338939813 1104 38-39 0.4858957933012107 1104 40-41 0.22820645812308982 1104 42-43 0.17446262494090092 1104 44-45 0.6793491277253736 1104 46-47 0.005872575022003446 1104 48-49 -0.0462128680409748 1104 50-51 -0.029834216659700985 1104 52-53 0.03290367068139943 1104 54-55 0.2716090369747022 1104 56-57 0.09093025975919033 1104 58-59 0.7672299913569276 1104 60-61 0.20457402530065139 1104 62-63 0.619163710070552 1104 64-65 0.04694387725810856 1104 66-67 0.006325206927343174 1104 68-69 0.29205127307569967 1104 70-71 -0.7343791531605248 1104 72-73 -1.3939707460801003 1104 74-75 -1.4935316676978196 1104 76-77 -0.9768302484619049 1104 78-79 -0.9968450057044258 1104 80-81 -0.5756004895214133 1104 82-83 -0.07559971858873382 1104 84-85 -0.5088252020336306 1104 86-87 -0.47446385014843173 1104 88-89 -0.4018518088692993 1104 90-91 -0.0019166813246869197 1104 92-93 -0.03737586239645552 1104 94-95 -0.28236917155715346 1104 96-97 -0.35305040045026814 1104 98-99 0.23101656566859674 1104 100-101 -0.1579380166911406 1105 1 0.27966058466280685 1105 2 0.2525271841769552 1105 3 0.3052404968039397 1105 4 0.18119394971461844 1105 5 -0.13816667962897355 1105 6 -0.2722058751647012 1105 7 -0.053379668898202226 1105 8 0.16664898176659904 1105 9 -0.059981395599713494 1105 10-11 0.18946369176639877 1105 12-13 -0.11594467433824462 1105 14-15 -0.17009440569410827 1105 16-17 -0.22793580187421014 1105 18-19 -0.313156584638314 1105 20-21 -0.4563164492127001 1105 22-23 -0.6349621464748907 1105 24-25 0.002723741707008287 1105 26-27 -0.07908302207815865 1105 28-29 0.02939497375970035 1105 30-31 -0.5011296071146774 1105 32-33 0.017247779594669055 1105 34-35 -0.34895699081068443 1105 36-37 -0.22220990233790872 1105 38-39 0.23680159040266346 1105 40-41 -0.15902180274647293 1105 42-43 0.18669088581046367 1105 44-45 0.044838258160154965 1105 46-47 -0.8116093090359655 1105 48-49 -0.12682881006996638 1105 50-51 0.45793752247073627 1105 52-53 0.5886101924205249 1105 54-55 0.3966090369747022 1105 56-57 1.0125788104838236 1105 58-59 0.3256539044004043 1105 60-61 0.5908964890687614 1105 62-63 0.515449941954607 1105 64-65 0.386164891750866 1105 66-67 1.0828650619998044 1105 68-69 2.2345331571336757 1105 70-71 2.160548383071358 1105 72-73 2.3922611379778758 1105 74-75 2.5839139844760943 1105 76-77 1.5924632297989625 1105 78-79 0.8052383276289099 1105 80-81 0.5362654525075747 1105 82-83 0.5593640495272112 1105 84-85 0.9970624791257912 1105 86-87 0.5187426715907009 1105 88-89 -0.057196011767853605 1105 90-91 0.2915615795448794 1105 92-93 0.9100879057194895 1105 94-95 0.7457105385877689 1105 96-97 0.3978553966511811 1105 98-99 0.1580998990019289 1105 100-101 -0.19688729205345723 1106 1 -0.2429843428734202 1106 2 -0.0463858593013029 1106 3 0.28984194607929936 1106 4 0.17938235551172 1106 5 0.34734056674783886 1106 6 0.32109122628457243 1106 7 0.24372178037715742 1106 8 -0.0027350762044164867 1106 9 0.11846063338579427 1106 10-11 -0.27158703287128105 1106 12-13 -0.4261801815846198 1106 14-15 -0.2244422317810617 1106 16-17 -0.06126913520753874 1106 18-19 0.41555718347762394 1106 20-21 -0.016551956459082362 1106 22-23 0.17165017236568758 1106 24-25 0.062053451851944885 1106 26-27 -0.20815910903468193 1106 28-29 -9.492291388468743E-4 1106 30-31 -0.04641946218713855 1106 32-33 -0.1231507711299713 1106 34-35 -0.11933742559329374 1106 36-37 0.16184806867659063 1106 38-39 0.17067840199685946 1106 40-41 -0.03266310709430087 1106 42-43 0.41359305972351024 1106 44-45 -0.8858682635789705 1106 46-47 0.17978561850026153 1106 48-49 0.388569740654674 1106 50-51 0.6984266529055176 1106 52-53 -0.11927024236208439 1106 54-55 -0.3370866151992118 1106 56-57 -0.050374088066895695 1106 58-59 -0.03711783473003294 1106 60-61 0.38935663399630727 1106 62-63 0.2387289274618567 1106 64-65 0.2752047468233272 1106 66-67 0.7671947721447339 1106 68-69 1.172486055684395 1106 70-71 2.1134469337959985 1106 72-73 1.8234205582677205 1106 74-75 1.4195118105630478 1106 76-77 1.6970827950163532 1106 78-79 1.2422854290781835 1106 80-81 1.2396169017829344 1106 82-83 1.1091828901069185 1106 84-85 1.3824791457924555 1106 86-87 1.0581448455037474 1106 88-89 0.7503221041741774 1106 90-91 0.5633007099796608 1106 92-93 0.8539284854296305 1106 94-95 1.380674306703714 1106 96-97 -0.026963443928529784 1106 98-99 1.2853643917555537 1106 100-101 1.3420619833088594 1107 1 0.5312631487653761 1107 2 0.3967579534077217 1107 3 -0.12905437499093608 1107 4 0.12670677022743604 1107 5 0.31055126908897535 1107 6 0.3704223299635032 1107 7 0.4594408439223088 1107 8 0.38940539202301494 1107 9 0.5666211685028557 1107 10-11 0.5917072815099829 1107 12-13 0.6540873769438136 1107 14-15 0.608751748152045 1107 16-17 0.26565394171554146 1107 18-19 0.493734441002708 1107 20-21 0.5252540636078109 1107 22-23 0.5918006740379269 1107 24-25 0.4746788699121396 1107 26-27 0.860820824075688 1107 28-29 0.5830808711956053 1107 30-31 0.7809216749366001 1107 32-33 1.1598759847228735 1107 34-35 1.4178699322662354 1107 36-37 0.973302918174916 1107 38-39 0.844173385274452 1107 40-41 0.3289589664843007 1107 42-43 0.7564024242719967 1107 44-45 1.3549344120063083 1107 46-47 1.1555381268614724 1107 48-49 0.028619907878756123 1107 50-51 0.8593798301630429 1107 52-53 1.2737063462666782 1107 54-55 1.3773782677439286 1107 56-57 1.1143416309966483 1107 58-59 1.390557750554251 1107 60-61 1.5436208480431262 1107 62-63 1.908879429134096 1107 64-65 1.6594020712380413 1107 66-67 2.160589420974162 1107 68-69 1.8635395673900845 1107 70-71 1.945804793327774 1107 72-73 2.110209855926591 1107 74-75 2.6536255229376344 1107 76-77 2.330443999029736 1107 78-79 2.753154994295574 1107 80-81 2.868797503789626 1107 82-83 2.942376870040029 1107 84-85 3.026709915023222 1107 86-87 2.4586465177445476 1107 88-89 2.591041167719329 1107 90-91 2.6281000410833393 1107 92-93 2.567139187770767 1107 94-95 2.6992361796134112 1107 96-97 2.7936887299845132 1107 98-99 3.086785796437823 1107 100-101 2.791894759228594 1108 1 0.20245470230986484 1108 2 0.06870365476518714 1108 3 0.10058363405883597 1108 4 0.07580179285186972 1108 5 0.0873235164494588 1108 6 0.2682353013058858 1108 7 0.04465954678806838 1108 8 0.1507176092175797 1108 9 0.03193036910617053 1108 10-11 0.23480682902129502 1108 12-13 0.35770728644607885 1108 14-15 0.4377487315607951 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0.6147024193590909 2316 48-49 0.7011032886954709 2316 50-51 0.8094035718534514 2316 52-53 -0.3770533878263862 2316 54-55 0.8302510122833482 2316 56-57 0.46358189690358387 2316 58-59 0.34532983032632814 2316 60-61 -0.05146462204234581 2316 62-63 -0.21564573705773427 2316 64-65 -0.2531097996071594 2316 66-67 0.2815533336047409 2316 68-69 -0.5390007934836163 2316 70-71 0.56795579047877 2316 72-73 0.8644833602000901 2316 74-75 0.9330652190439928 2316 76-77 0.8960897730088391 2316 78-79 0.9167352412091567 2316 80-81 0.9116512549767073 2316 82-83 0.48760479026795167 2316 84-85 0.5337137136936896 2316 86-87 0.7390358814672453 2316 88-89 1.5766774450222734 2316 90-91 0.8409442955942623 2316 92-93 1.1739767946083752 2316 94-95 0.5859883163655475 2316 96-97 0.6933800880092029 2316 98-99 0.8297819977673626 2316 100-101 -0.08346136612753341 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 2.0 14 10.0 15 28.0 16 91.0 17 277.0 18 506.0 19 635.0 20 797.0 21 1023.0 22 1125.0 23 909.0 24 733.0 25 612.0 26 690.0 27 750.0 28 917.0 29 1108.0 30 852.0 31 762.0 32 881.0 33 1062.0 34 1544.0 35 4202.0 36 4209.0 37 8667.0 38 17892.0 39 4894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.489709245344656 23.430977530944862 15.795491669389088 19.28382155432139 2 15.956868430590793 24.675607104023197 40.99673794853207 18.370786516853933 3 15.804639362087713 30.396882928597318 37.50271837622327 16.2957593330917 4 9.139180862631388 24.960130482058716 40.65059804276912 25.250090612540777 5 8.417905038057267 40.93693367162015 38.845596230518304 11.799565059804278 6 20.735773831098225 43.55744835085176 19.309532439289598 16.39724537876042 7 18.702428416092786 40.11779630300833 24.6339253352664 16.545849945632476 8 22.084088437839796 36.23776730699529 23.689742660384198 17.98840159478072 9 23.82384922073215 16.03117071402682 24.708227618702427 35.4367524465386 10-11 20.19572308807539 29.188111634650237 31.593874592243566 19.022290685030807 12-13 22.369517941283075 29.641174338528455 31.07647698441464 16.91283073577383 14-15 19.914824211670894 30.057992026096414 30.475715839072127 19.551467923160565 16-17 16.384559623051832 33.92080463936209 31.379122870605293 18.31551286698079 18-19 15.996737948532077 30.09061254077564 36.54041319318594 17.372236317506342 20-21 16.296665458499458 29.273287422979337 37.05509242479159 17.374954693729613 22-23 16.537694816962667 30.492026096411745 36.6699891264951 16.30028996013048 24-25 16.566690830010874 31.632837984777098 37.03787604204422 14.762595143167815 26-27 16.410837259876768 30.711308445088804 37.707502718376226 15.17035157665821 28-29 14.839615802827112 29.25063428778543 39.4445451250453 16.465204784342152 30-31 15.895251902863356 29.41826748822037 37.34233417905038 17.34414642986589 32-33 16.400869880391447 29.785248278361724 38.75951431678144 15.054367524465388 34-35 16.25226531351939 31.47789054005074 37.380391446176155 14.889452700253717 36-37 15.898876404494382 30.534613990576297 36.62377673069953 16.942732874229794 38-39 15.062522653135193 30.946901051105474 37.20913374411018 16.781442551649146 40-41 15.074302283436028 30.050743022834357 38.75951431678144 16.11544037694817 42-43 16.300437662537718 30.130755081144265 37.59910836451944 15.969698891798584 44-45 15.50289960130482 31.195179412830736 37.30427691192461 15.997644073939835 46-47 17.08318231243204 30.189380210221096 35.16400869880392 17.56342877854295 48-49 16.56684094636595 30.084542266602632 35.325619115794815 18.022997671236602 50-51 15.025371511417179 30.193004711852122 36.03207683943458 18.74954693729612 52-53 15.260964117433854 31.361906487857922 36.85846321130845 16.518666183399784 54-55 15.52283436027546 30.714932946719824 35.43856469735411 18.3236679956506 56-57 16.967198260239215 31.34650235592606 33.74411018484958 17.94218919898514 58-59 15.425878941645523 31.314787966654585 35.95143167814426 17.307901413555633 60-61 17.01159840521928 32.88872779992751 33.176875679594055 16.922798115259152 62-63 15.444907575208408 33.65621602029721 33.46321130844509 17.435665096049295 64-65 15.16763320043494 33.683399782529904 32.98296484233418 18.166002174700978 66-67 15.343421529539688 33.40250090612541 32.85791953606379 18.396158028271113 68-69 15.695904313156941 32.67669445451251 32.3523015585357 19.275099673794855 70-71 15.695904313156941 32.752808988764045 33.25027183762232 18.30101486045669 72-73 16.632837984777094 31.323849220732146 32.801739760782894 19.241573033707866 74-75 15.771112722000725 31.47698441464299 32.61689017760058 20.13501268575571 76-77 15.614353026458863 31.930047118521205 33.03733236679957 19.41826748822037 78-79 16.2042406669083 31.1127220007249 32.848858281986224 19.834179050380573 80-81 15.902500906125407 30.87712939470823 32.69028633562885 20.530083363537514 82-83 15.860819137368612 31.024827836172523 32.68031895614353 20.43403407031533 84-85 15.5636100036245 30.372417542587893 32.73378035520116 21.330192098586444 86-87 15.887096774193548 30.65059804276912 32.60873504893077 20.853570134106562 88-89 16.329285973178685 30.193004711852122 32.360456687205506 21.117252627763683 90-91 15.893583667847661 29.668626935727943 33.08293841009795 21.354850986326444 92-93 16.21692642261689 29.83417905038057 32.46919173613628 21.479702790866256 94-95 16.044762595143165 28.698803914461763 33.34179050380573 21.914642986589346 96-97 16.362812613265675 28.796665458499454 32.56705328017397 22.27346864806089 98-99 15.466654584994563 29.06940920623414 32.16745197535339 23.296484233417907 100-101 15.55364262413918 28.692461036607465 32.99474447263501 22.75915186661834 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1580.0 1 935.0 2 317.0 3 350.0 4 243.0 5 162.5 6 272.5 7 527.5 8 737.5 9 718.0 10 439.0 11 187.5 12 173.0 13 174.0 14 148.0 15 137.0 16 155.0 17 169.5 18 172.5 19 182.5 20 202.0 21 229.0 22 243.5 23 286.5 24 326.5 25 372.0 26 524.0 27 695.0 28 772.5 29 879.5 30 1082.5 31 1238.5 32 1502.0 33 1839.0 34 2143.0 35 2434.0 36 2646.0 37 2771.5 38 2734.0 39 2626.5 40 2392.0 41 2166.5 42 2036.0 43 1934.0 44 1833.0 45 1684.5 46 1413.0 47 1132.5 48 1065.0 49 999.0 50 949.0 51 918.0 52 787.0 53 577.0 54 434.5 55 380.5 56 321.0 57 289.0 58 265.5 59 203.5 60 157.5 61 137.5 62 112.0 63 92.5 64 62.0 65 48.0 66 44.5 67 30.5 68 23.0 69 19.0 70 22.0 71 23.0 72 16.5 73 12.5 74 9.5 75 8.5 76 11.0 77 9.5 78 4.0 79 4.5 80 3.5 81 1.5 82 2.5 83 1.5 84 0.5 85 2.0 86 2.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1486045668720551 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 9.061254077564335E-4 44-45 0.0 46-47 0.0 48-49 9.061254077564335E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 9.061254077564335E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 55180.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.84015947807177 #Duplication Level Percentage of deduplicated Percentage of total 1 71.11795446061964 27.62232693004712 2 11.972751026502426 9.300471185212034 3 5.258491974617394 6.1272200072490035 4 2.944195595371407 4.574121058354477 5 1.8803658081373649 3.651685393258427 6 1.2971257932064204 3.0228343602754624 7 0.9471817842478537 2.5752084088437837 8 0.7418812989921613 2.305183037332367 9 0.5692422545726017 1.989851395433128 >10 3.0888391190742817 21.785067053280173 >50 0.11664800298618887 3.1152591518666184 >100 0.03732736095558044 3.497644073939833 >500 0.01866368047779022 6.379122870605292 >1k 0.00933184023889511 4.0540050743022835 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 1134 2.0550924247915914 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1103 1.9989126495106924 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1000 1.812250815512867 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 983 1.7814425516491483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 972 1.7615077926785068 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 565 1.0239217107647698 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 432 0.7828923523015585 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 319 0.5781080101486046 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 273 0.4947444726350127 RNA PCR Primer, Index 26 (100% over 22bp) GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 270 0.48930772018847407 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 200 0.3624501631025734 No Hit ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 190 0.3443276549474447 RNA PCR Primer, Index 26 (100% over 24bp) GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.2428416092787242 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 0.2029720913374411 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 99 0.17941283073577383 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 90 0.16310257339615802 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 90 0.16310257339615802 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 83 0.15041681768756796 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 81 0.14679231605654222 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 80 0.14498006524102935 RNA PCR Primer, Index 26 (100% over 29bp) TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 73 0.13229430953243929 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 72 0.13048205871692642 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 72 0.13048205871692642 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 72 0.13048205871692642 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 71 0.12866980790141355 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67 0.1214208046393621 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 66 0.11960855382384922 RNA PCR Primer, Index 26 (100% over 27bp) CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 66 0.11960855382384922 No Hit GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG 65 0.11779630300833635 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 62 0.11235955056179776 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 62 0.11235955056179776 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 59 0.10692279811525915 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 59 0.10692279811525915 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 57 0.10329829648423343 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 57 0.10329829648423343 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.061254077564335E-4 0.0 20-21 0.0 0.0 0.0 0.0027183762232693004 0.0 22-23 0.0 0.0 0.0 0.006342877854295034 0.0 24-25 0.0 0.0 0.0 0.014498006524102935 0.0 26-27 0.0 0.0 0.0 0.035338890902500905 0.0 28-29 0.0 0.0 0.0 0.08336353751359188 0.0 30-31 0.0 0.0 0.0 0.19028633562885103 0.0 32-33 0.0 0.0 0.0 0.2836172526277637 0.0 34-35 0.0 0.0 0.0 0.3814787966654585 0.0 36-37 0.0 0.0 0.0 0.5264588619064878 0.0 38-39 0.0 0.0 0.0 0.7276187024284161 0.0 40-41 0.0 0.0 0.0 1.0067053280173976 0.0 42-43 0.0 0.0 0.0 1.1888365349764407 0.0 44-45 0.0 0.0 0.0 1.3401594780717652 0.0 46-47 0.0 0.0 0.0 1.4996375498368972 0.0 48-49 0.0 0.0 0.0 1.6500543675244654 0.0 50-51 0.0 0.0 0.0 1.7923160565422254 0.0 52-53 0.0 0.0 0.0 1.9445451250453063 0.0 54-55 0.0 0.0 0.0 2.1212395795578107 0.0 56-57 0.0 0.0 0.0 2.369517941283074 0.0 58-59 0.0 0.0 0.0 2.6023921710764766 0.0 60-61 0.0 0.0 0.0 2.8243928959768034 0.0 62-63 0.0 0.0 0.0 3.045487495469373 0.0 64-65 0.0 0.0 0.0 3.2575208408843785 0.0 66-67 0.0 0.0 0.0 3.5357013410656037 0.0 68-69 0.0 0.0 0.0 3.843783979702791 0.0 70-71 0.0 0.0 0.0 4.226168901776006 0.0 72-73 0.0 0.0 0.0 4.596774193548387 0.0 74-75 0.0 0.0 0.0 5.02808988764045 0.0 76-77 0.0 0.0 0.0 5.431315694092062 0.0 78-79 0.0 0.0 0.0 5.865349764407394 0.0 80-81 0.0 0.0 0.0 6.312975715839072 0.0 82-83 0.0 0.0 0.0 6.761507792678507 0.0 84-85 0.0 0.0 0.0 7.254440014498007 0.0 86-87 0.0 0.0 0.0 7.790866255889815 0.0 88-89 0.0 0.0 0.0 8.327292497281624 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATGAC 15 6.585026E-4 94.99728 8 GATGACC 20 0.0020638243 71.24796 9 CCACCTA 20 0.0020638243 71.24796 8 ATGGGAT 50 4.1687417E-7 56.998367 5 CACCTAT 25 0.004996598 56.998367 9 ATCCACC 25 0.004996598 56.998367 6 GGTATCA 515 0.0 50.865047 1 CGCAGAA 70 7.740164E-8 47.49864 9 TTAACTG 25 0.0016097154 37.99891 60-61 GTATCAA 755 0.0 34.601658 2 CATGGGA 180 0.0 34.304573 4 GTTATTG 30 0.0039361706 31.66576 82-83 AACGCAG 880 0.0 29.686651 7 GTATAAT 65 0.0057206117 29.30965 1 CTTGAAA 265 0.0 28.678425 56-57 CAACGCA 925 0.0 28.242435 6 TTGAAAA 270 0.0 28.147345 58-59 TCAACGC 935 0.0 27.940376 5 TATCAAC 940 0.0 27.791758 3 GACCCAC 35 0.008360464 27.142078 12-13 >>END_MODULE