FastQCFastQC Report
Fri 10 Feb 2017
SRR2031864_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031864_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954009
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35900.18372484466550565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.1622305731447501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40450.042.3918381
GTATCAA61650.034.9026761
TCAACGC75800.027.8858854
ATCAACG76850.027.6903063
TATCAAC77450.027.598452
CAACGCA77250.027.485445
AACGCAG78900.026.9106456
GTATAGC8050.026.5527951
GTACATG77000.024.8603881
TACATGG76900.024.0897252
ACGCAGA88750.023.9774677
TAGTACG1401.7803086E-523.754
CGCAGAG92550.023.04438
GTATTAG4400.022.6704541
ACATGGG82000.021.9542683
GAGTACT39600.021.7108612-13
TAGCCCT20100.021.5049764
GTATAAT4650.021.4516121
GTACTTT39450.021.37198814-15
GCAGAGT100750.020.7444159
Produced by FastQC (version 0.11.5)