FastQCFastQC Report
Fri 10 Feb 2017
SRR2031864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954009
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46650.238739944391249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39040.1997943714691181No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT23570.12062380470100188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.11003019945148666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42150.045.363281
GTATCAA65350.034.644741
GTATAGC8500.029.0978161
TCAACGC79850.028.0177064
TATCAAC80950.027.695662
CAACGCA80850.027.6711675
ATCAACG80750.027.6466123
AACGCAG83450.026.9797926
TATAGCC10400.026.9466782
GTACATG75350.024.807631
TACATGG75850.024.7359222
ACGCAGA95900.023.2790817
ACATGGG78950.022.982533
GTATATA4800.022.7909561
CGCAGAG97900.022.7064748
ATAGCCC11400.022.0829773
GAGTACT50000.022.03964212-13
GTACTTT50250.021.59915414-15
GCAGAGT107650.020.296939
GTATTAC2851.9335857E-920.0268381
Produced by FastQC (version 0.11.5)