FastQCFastQC Report
Fri 10 Feb 2017
SRR2031863_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031863_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662312
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33610.2021882775315344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.1769824196661036No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.049.0573771
GTATCAA31950.040.2895131
TCAACGC33700.037.2106824
ATCAACG34850.035.8464853
CAACGCA35400.035.826275
AACGCAG38100.033.28746
TATCAAC39200.032.2321432
CGCAGAG41900.029.9284028
ACGCAGA42200.029.603087
TATAGCG1151.2820895E-424.7826085
GCAGAGT53450.022.7502339
TACATGG24700.021.730772
GAGTACT38900.021.18573412-13
GTACATG26000.020.8269251
CAGAGTA51350.020.21178210-11
ACATGGG25800.019.883723
AGAGTAC47000.019.55585110-11
GTACTTT45600.018.12500214-15
AGTACTT43850.017.5484612-13
CATGGGG17200.017.1220934
Produced by FastQC (version 0.11.5)