FastQCFastQC Report
Fri 10 Feb 2017
SRR2031863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662312
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39630.2384028990947548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34300.20633912286020917No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT26340.15845400863375828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18990.1142384823065706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.046.003831
GTATCAA37800.038.768321
TCAACGC40450.035.5803344
CAACGCA40600.035.2148935
ATCAACG40800.034.809433
AACGCAG42450.033.6802066
TATCAAC45400.031.5963552
CGCAGAG47150.030.1214188
ACGCAGA47400.029.962557
GCAGAGT59550.023.769529
GAGTACT43150.021.74063512-13
AGAGTAC51900.021.00396210-11
CAGAGTA56950.020.97636210-11
GTACATG26850.019.3152471
TACATGG25950.018.6701892
GTACTTT50900.018.4770814-15
GCACCGT2152.3958337E-517.6741056
AGTACTT50750.017.59574712-13
GCGAGCG1350.00940307217.5922819
ACCGTGC2002.7616348E-416.6247068
Produced by FastQC (version 0.11.5)